Exacerbation of colon carcinogenesis by Blastocystis sp.

Kumarasamy, V. and Kuppusamy, U.R. and Jayalakshmi, P. and Samudi, C. and Ragavan, N.D. and Kumar, S. (2017) Exacerbation of colon carcinogenesis by Blastocystis sp. PLoS ONE, 12 (8). e0183097. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0183097.

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Official URL: http://dx.doi.org/10.1371/journal.pone.0183097


Colorectal cancer (CRC) is one the most commonly diagnosed cancers worldwide and the number is increasing every year. Despite advances in screening programs, CRC remains as the second leading cause of cancer deaths in the United States. Oxidative stress plays an important role in the molecular mechanisms of colorectal cancer (CRC) and has been shown to be associated with Blastocystis sp., a common intestinal microorganism. In the present study, we aimed to identify a role for Blastocystis sp. in exacerbating carcinogenesis using in vivo rat model. Methylene blue staining was used to identify colonic aberrant crypt foci (ACF) and adenomas formation in infected rats whilst elevation of oxidative stress bio-marker levels in the urine and serum samples were evaluated using biochemical assays. Histological changes of the intestinal mucosa were observed and a significant number of ACF was found in Blastocystis sp. infected AOM-rats compared to the AOM-controls. High levels of urinary oxidative indices including advanced oxidative protein products (AOPP) and hydrogen peroxide were observed in Blastocystis sp. infected AOM-rats compared to the uninfected AOM-rats. Our study provides evidence that Blastocystis sp. has a significant role in enhancing AOM-induced carcinogenesis by resulting damage to the intestinal epithelium and promoting oxidative damage in Blastocystis sp. infected rats.

Item Type: Article
Funders: University Malaya High Impact Research Grant UM.C/625/1/HIR/MOHE/MED/44
Uncontrolled Keywords: Aberrant Crypt Foci; Animals; Biomarkers, Tumor; Blastocystis; Carcinogenesis; Colorectal Neoplasms; Disease Models, Animal; Humans; Oxidative Stress; Precancerous Conditions; Rats; Rats, Inbred F344
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 06 Sep 2018 01:19
Last Modified: 06 Sep 2018 01:19
URI: http://eprints.um.edu.my/id/eprint/19132

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