Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

Sebayang, A.H. and Masjuki, Haji Hassan and Ong, H.C. and Dharma, S. and Silitonga, A.S. and Kusumo, F. and Mahlia, T.M.I. and Bahar, A.H. (2017) Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology. Energies, 10 (1). p. 35. ISSN 1996-1073, DOI https://doi.org/10.3390/en10010035.

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Official URL: http://dx.doi.org/10.3390/en10010035


Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock.

Item Type: Article
Funders: Ministry of Education of Malaysia and University of Malaya, Kuala Lumpur, Malaysia, under the High Impact Research Grant (Project title: Clean diesel technology for military and civilian transport vehicles, Grant No.: UM.C/HIR/MOE/ENG/07 D000007-16001), University Malaya Research Grant Scheme (Grant No.: RP022A-13AET), SATU Joint Research Scheme (RU021A-2015), Postgraduate Research Grant, PPP (Grant No.: PG014-2015A)
Uncontrolled Keywords: Manihot glaziovii (M. glaziovii); Optimization; Hydrolysis; Fermentation; Bioethanol; Alternative fuel
Subjects: T Technology > TJ Mechanical engineering and machinery
Divisions: Faculty of Engineering
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 30 Aug 2018 04:37
Last Modified: 16 Oct 2018 05:22
URI: http://eprints.um.edu.my/id/eprint/19035

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