In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm

Zahari, Azeana and Ablat, A. and Sivasothy, Y. and Mohamad, Jamaludin and Choudhary, M.I. and Awang, Khalijah (2016) In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm. Asian Pacific Journal of Tropical Medicine, 9 (4). pp. 328-332. ISSN 1995-7645, DOI

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Objective: To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri. Methods: Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography. The antiplasmodial activity of the isolated compounds was evaluated using the histidine-rich protein II assay. The isolated alkaloids were also tested for their antioxidant activity using three different assays; DPPH, ferric reducing ability of plasma and metal chelating assays. Results: Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoptosis. The antioxidant properties of the alkaloids under investigation revealed that in addition to the antiplasmodial activity, the alkaloids could also prevent oxidative stress. (+)-laurotetanine and (+)-norstephasubine exhibited strong antiplasmodial activities with IC50 values of 0.189 and 0.116 μM, respectively. Conclusions: Interestingly, the two most potent compounds that exhibit antiplasmodial activity also exhibit good antioxidant activities. The crude dichloromethane extract and the isolated compounds exert substantial antiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host.

Item Type: Article
Funders: University of Malaya Research Grant (RP001/2012A), (RP001/2012B), Postgraduate Research Funds of University of Malaya (PV050/2012A)
Uncontrolled Keywords: Bisbenzylisoquinoline; Laurotetanine; Norstephasubine; Antiplasmodial; Antioxidant; Oxidative stress
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 May 2018 04:03
Last Modified: 01 Mar 2019 09:13

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