Invasion characteristics of a Plasmodium knowlesi line newly isolated from a human

Amir, A. and Russell, B. and Liew, J.W.K. and Moon, R.W. and Fong, M.Y. and Vythilingam, I. and Subramaniam, V. and Snounou, G. and Lau, Y.L. (2016) Invasion characteristics of a Plasmodium knowlesi line newly isolated from a human. Scientific Reports, 6. p. 24623. ISSN 2045-2322, DOI

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Plasmodium knowlesi is extensively used as an important malaria model and is now recognized as an important cause of human malaria in Malaysia. The strains of P. knowlesi currently used for research were isolated many decades ago, raising concerns that they might no longer be representative of contemporary parasite populations. We derived a new P. knowlesi line (University Malaya line, UM01), from a patient admitted in Kuala Lumpur, Malaysia, and compared it with a human-adapted laboratory line (A1-H.1) derived from the P. knowlesi H strain. The UM01 and A1-H.1 lines readily invade human and macaque (Macaca fascicularis) normocytes with a preference for reticulocytes. Whereas invasion of human red blood cells was dependent on the presence of the Duffy antigen/receptor for chemokines (DARC) for both parasite lines, this was not the case for macaque red blood cells. Nonetheless, differences in invasion efficiency, gametocyte production and the length of the asexual cycle were noted between the two lines. It would be judicious to isolate and characterise numerous P. knowlesi lines for use in future experimental investigations of this zoonotic species.

Item Type: Article
Uncontrolled Keywords: Animals; Erythrocytes; Female; Humans; Macaca; Malaria; Plasmodium knowlesi; Young Adult
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 16 Nov 2017 03:53
Last Modified: 16 Nov 2017 03:53

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