Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

Osahor, A. and Deekonda, K. and Lee, C.W. and Sim, E.U.H. and Radu, A. and Narayanan, K. (2017) Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis. Analytical Biochemistry, 534. pp. 46-48. ISSN 0003-2697, DOI https://doi.org/10.1016/j.ab.2017.07.008.

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Official URL: https://doi.org/10.1016/j.ab.2017.07.008


Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. This protocol is significantly cheaper and easier to adapt compared to conventional methods, and will allow routine preparation/viewing of eukaryotic or bacterial samples for basic morphological studies.

Item Type: Article
Funders: MOSTI eScience grant 02-02-10-SF0252 from the Ministry of Science, Technology and Innovation, Malaysia, Higher Degree by Research (HDR) Scholarship from Monash University Malaysia
Uncontrolled Keywords: Cells; Dehydration; DNA; Escherichia coli; HeLa; Microscopy osmium tetroxide
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 31 Jul 2017 06:28
Last Modified: 31 Jul 2017 06:28
URI: http://eprints.um.edu.my/id/eprint/17581

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