Environmental factors influencing distribution of archaea in tropical coastal waters

Suet, Y.S. and Choon, W.L. (2012) Environmental factors influencing distribution of archaea in tropical coastal waters. In: 2nd Conference for Regional Cooperation in Ocean and Earth Science Research in the South China Sea, 21-24 October 2012, University of Malaya, Kuala Lumpur, Malaysia.

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We explore the archaea diversity in four locations along the Straits of Malacca, which is a semi-enclosed narrow tropical sea that connects the Indian Ocean to the South China Sea. Samples from these different environments were collected and four 16S rONA clone libraries were constructed with archaea specific primers (Arc21 F and Arc958R). In total, 1074 randomly selected clones were screened by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis using three different restriction enzymes (Rsal, Cfol, and DdeI). Archaeal operational taxonomic units (OTUs) were defined as sequences which are 297% similar. Diversity index were determined for each clone library which has a Good's coverage of more than 85%. Relationship between OTUs with environmental variables were analysed with canonical correspondence analysis (CCA). Majority of the sequences from three clone libraries were affiliated to Euryarchaeota, except for one clone library which were dominated by Thaumarchaeota sequences. This could be due to the higher chlorophyll a concentrations. Sequences obtained indicate that tropical waters along the west coast of Peninsular Malaysia harbour a rich diversity of Archaea.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Archaea; Straits of malacca; Environment; Clone
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science
Depositing User: Mr. Mohd Safri
Date Deposited: 15 Dec 2015 06:24
Last Modified: 15 Dec 2015 06:24
URI: http://eprints.um.edu.my/id/eprint/15059

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