Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers

Lim, Wei Yin and Goh, Choon Hian and Thevarajah, T. Malathi and Goh, Boon Tong and Khor, Sook Mei (2020) Using SERS-based microfluidic paper-based device (μPAD) for calibration-free quantitative measurement of AMI cardiac biomarkers. Biosensors and Bioelectronics, 147. p. 111792. ISSN 0956-5663, DOI

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Recently, surface enhanced Raman scattering (SERS) has attracted much attention in medical diagnosis applications owing to better detection sensitivity and lower limit of detection (LOD) than colorimetric detection. In this paper, a novel calibration-free SERS−based μPAD with multi-reaction zones for simultaneous quantitative detection of multiple cardiac biomarkers — GPBB, CK−MB and cTnT for early diagnosis and prognosis of acute myocardial infarction (AMI) are presented. Three distinct Raman probes were synthesised, subsequently conjugated with respective detecting antibodies and used as SERS nanotags for cardiac biomarker detection. Using a conventional calibration curve, quantitative simultaneous measurement of multiple cardiac biomarkers on SERS-based μPAD was performed based on the characteristic Raman spectral features of each reporter used in different nanotags. However, a calibration free point-of-care testing device is required for fast screening to rule-in and rule-out AMI patients. Partial least squares predictive models were developed and incorporated into the immunosensing system, to accurately quantify the three unknown cardiac biomarkers levels in serum based on the previously obtained Raman spectral data. This method allows absolute quantitative measurement when conventional calibration curve fails to provide accurate estimation of cardiac biomarkers, especially at low and high concentration ranges. Under an optimised condition, the LOD of our SERS-based μPAD was identified at 8, 10, and 1 pg mL−1, for GPBB, CK−MB and cTnT, respectively, which is well below the clinical cutoff values. Therefore, this proof-of-concept technique shows significant potential for highly sensitive quantitative detection of multiplex cardiac biomarkers in human serum to expedite medical decisions for enhanced patient care. © 2019 Elsevier B.V.

Item Type: Article
Funders: Impact Oriented Interdisciplinary Research Grant ( IIRG020A-2019 ), Research University Grant ( GPF057B-2018 ), University Malaya Research Grant (UMRG) Programme−Frontier Science ( RP038C-17AFR )
Uncontrolled Keywords: Paper-based microfluidic device; Surface enhanced Raman scattering; Acute myocardial infarction; Cardiac biomarkers; Multiplex detection; Multivariate analysis
Subjects: Q Science > QC Physics
Q Science > QD Chemistry
R Medicine
Divisions: Faculty of Engineering
Faculty of Medicine
Faculty of Science > Department of Chemistry
Faculty of Science > Department of Physics
Depositing User: MR Faizal II H
Date Deposited: 20 Nov 2015 08:54
Last Modified: 11 Nov 2019 04:45

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