Investigation of extraction and transesterification of algae by immobilized lipase as biofuel

Mohd Yusof, Noraini and Ong, Hwai Chyuan and Jan, Badrul Mohamed and Chong, Wen Tong and Masjuki, Haji Hassan (2015) Investigation of extraction and transesterification of algae by immobilized lipase as biofuel. In: 5th International Conference on Algal Biomass, Biofuels & Bioproducts, 7-10 June 2015, San Diego, USA. (Submitted)

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In this study, three different methods were investigated to study the disruption of algae cells walls such as ultrasonication, soxhlet and hexane (solvent) extraction. Chlorella sp. and Spirulina sp. were two selected algae species to analyze the effect of each method for biodiesel production. The oil yield is calculated to determine the best effective method for oil extraction. The highest oil yield obtained by ultrasonication followed by Soxhlet and hexane extraction. Moreover, acid value for Chlorella is the lowest for all extraction method. Then, the crude algae oil was transesterified by Novozyme 435, an immobilized enzyme, at 60°C, 300 rpm for 24 hours to hydrolyze triglycerides into free fatty acids. The ester content was analyzed by FTIR and found at 1735, 1731, 1214, 1162, 1161, 1064, 1060, 1059 cm-1 frequency. Fatty acid derivatives were measured by gas chromatography and palmitic acid was among ofthe major component in microalgae biodiesel.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Investigation; Extraction; Transesterification; Algae; Immobilized lipase; Biofuel
Subjects: T Technology > TJ Mechanical engineering and machinery
T Technology > TP Chemical technology
Divisions: Faculty of Engineering
Depositing User: Mr. Mohd Samsul Ismail
Date Deposited: 21 Sep 2015 00:27
Last Modified: 18 Oct 2018 01:06

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