Rapid and sensitive detection of Salmonella with reduced graphene oxide-carbon nanotube based electrochemical aptasensor

Appaturi, Jimmy Nelson and Pulingam, Thiruchelvi and Thong, Kwai Lin and Muniandy, Shalini and Ahmad, Noraini and Leo, Bey Fen (2020) Rapid and sensitive detection of Salmonella with reduced graphene oxide-carbon nanotube based electrochemical aptasensor. Analytical Biochemistry, 589. p. 113489. ISSN 0003-2697, DOI https://doi.org/10.1016/j.ab.2019.113489.

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Official URL: https://doi.org/10.1016/j.ab.2019.113489


Rapid detection of foodborne pathogens is crucial as ingestion of contaminated food products may endanger human health. Thus, the objective of this study was to develop a biosensor using reduced graphene oxide-carbon nanotubes (rGO-CNT) nanocomposite via the hydrothermal method for accurate and rapid label-free electrochemical detection of pathogenic bacteria such as Salmonella enterica. The rGO-CNT nanocomposite was characterized using Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction and transmission electron microscopy. The nanocomposite was dropped cast on the glassy carbon electrode and further modified with amino-modified DNA aptamer. The resultant ssDNA/rGO-CNT/GCE aptasensor was then used to detect bacteria by using differential pulse voltammetry (DPV) technique. Synergistic effects of aptasensor was evident through the combination of enhanced electrical properties and facile chemical functionality of both rGO and CNT for the stable interface. Under optimal experimental conditions, the aptasensor could detect S. Typhimurium in a wide linear dynamic range from 101 until 108 cfu mL−1 with a 101 cfu mL−1 of the limit of detection. This aptasensor also showed good sensitivity, selectivity and specificity for the detection of microorganisms. Furthermore, we have successfully applied the aptasensor for S. Typhimurium detection in real food samples. © 2019 Elsevier Inc.

Item Type: Article
Funders: University of Malaya Research Grant - UMRG ( RP045D-17AET ), Trans-disciplinary Research Grant Scheme (TRGS) under Grant number TR002A-2014B, TR002B-2014B and TR002C-2014B
Uncontrolled Keywords: Aptasensor; Aptamer; Reduced graphene oxide; Carbon nanotubes; Salmonella
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine
Divisions: Faculty of Medicine
Faculty of Science > Department of Chemistry
Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Mr Faizal 2
Date Deposited: 25 Mar 2015 02:43
Last Modified: 11 Nov 2019 01:33
URI: http://eprints.um.edu.my/id/eprint/13114

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