Optimal conditions for cold-shock induction of triploidy in red tilapia

Padmaja, J.P. and Srijaya, T.C. and Anuar, H. and Anil, K.C. and Boonsirm, W. and Andrew, J. (2014) Optimal conditions for cold-shock induction of triploidy in red tilapia. Aquaculture International, 22 (3). pp. 1163-1174. ISSN 0967-6120, DOI https://doi.org/10.1007/s10499-013-9736-4.


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Production of sterile triploid red tilapia [Oreochromis mossambicus (Mozambique tilapia); Peters, 1852 x Oreochromis niloticus (Nile tilapia); Linnaeus, 1758] is an effective strategy to overcome their prolific breeding. Optimal conditions for cold-shock induction of triploidy in red tilapia were investigated by experimentally examining two variables: appropriate temperature of the shock and duration of shock treatment. A constant time after insemination of 4 min was used to determine the best combination of temperature (6, 7, 8, 9, 11, 13, 15 A degrees C) with different durations of shock (10, 20, 30, 40, 50 min) with resultant ploidy level verified karyotypically. Shock duration for 30 min at a temperature of 9 A degrees C was found most effective in producing maximum triploidy (98.7 %) with higher rates of hatching (63.2 %) and survival up to yolk-sac stage (75.8 %). The chromosome count confirmed that triploid percentages were higher when cold shock was used for longer durations at each temperature; however, hatching rates were generally decreased. The maximum triploid yield (82.1 %) obtained was higher than the yield obtained using heat shock (72.7 %) in red tilapia previously. The application of the results of this study has the potential to greatly improve the production of triploid red tilapia in commercial aquaculture.

Item Type: Article
Uncontrolled Keywords: Chromosome Manipulation; Sterile Tilapia; Sterile Stock; Red Tilapia; Triploidy Induction; Turbot Scophthalmus-Maximus; Oreochromis-Niloticus L.; Dicentrarchus-Labrax L; Nile Tilapia; Heat-Shock; Sea Bass; Fish; Growth; Performance; Aquaculture
Subjects: R Medicine > RK Dentistry
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 19 Jun 2014 08:21
Last Modified: 06 Jan 2015 03:24
URI: http://eprints.um.edu.my/id/eprint/10639

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