High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene

Iqbal, Asma and Lim, Yvonne Ai Lian and Surin, Johari and Sim, Benedict Lim Heng (2012) High diversity of Cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene. PLoS ONE, 7 (2). e31139. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0031139.

High_Diversity_of_Cryptosporidium_Subgenotypes_Identified_in_Malaysian_HIV_AIDS_Individuals_Targeting_gp60_Gene.pdf - Published Version

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Official URL: https://doi.org/10.1371/journal.pone.0031139


Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients. Methodology/Principal Findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2% of 346) isolates were determined as Cryptosporidium positive with 72.2% (of 18) identified as Cryptosporidium parvum whilst 27.7% as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates). Conclusions/Significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals.

Item Type: Article
Funders: University of Malaya (Research grant No. HIR J-00000-73587 and FS 196/2008B)
Uncontrolled Keywords: Cryptosporidium parvum; Cardiobacterium hominis; Cryptosporidium; Opportunistic infections; HIV infections; HIV; Polymerase chain reaction; Sequence alignment
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Ms. Nur Azzura Azzahra Jaafar
Date Deposited: 13 Jun 2014 01:21
Last Modified: 02 Dec 2019 03:53
URI: http://eprints.um.edu.my/id/eprint/10547

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