Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma

Siow, M.Y. and Ng, L.P.K. and Chong, V.K.V. and Jamaludin, M. and Abraham, M.T. and Rahman, Z.A.A. and Kallarakkal, T.G. and Yang, Y.H. and Cheong, S.C. and Zain, R.B. (2014) Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma. Oral Diseases, 20 (4). pp. 345-361. DOI https://doi.org/10.1111/odi.12118.


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Objectives To identify differentially expressed miRNA between oral squamous cell carcinoma (OSCC) and non-cancer (NC) and to associate these with clinico-pathological parameters. Materials and methods miRNA microarray profiling was utilized to obtain the expression profile of miRNAs in four OSCC and four NC samples. The expression of miR-31 and miR-375 was further validated in 26 OSCC and three NC samples using real-time-PCR. The association between miRNA expression and clinico-pathological parameters was tested by univariate and multivariate analyses. Results Microarray profiling demonstrated that 15 and four miRNAs were up-regulated and down-regulated, respectively, in OSCC as compared with NC. miR-31 and miR-375 were validated as up- and down-regulated miRNAs, respectively. In univariate analyses, expression of miR-31 was significantly elevated in early stage, tumours with no metastatic nodes and those from the buccal mucosa. By contrast, low miR-375 expression was significantly associated with late stage disease, larger tumour size and the non-cohesive type of pattern of invasion in OSCC. The association between miR-31 expression with tumour staging and site and miR-375 with tumour staging remained significant in multivariate analyses. Conclusions This study has identified 19 miRNAs significantly associated with OSCC, and expressions of miR-31 and miR-375 were significantly related with clinico-pathological parameters suggesting they could be important in driving oral tumourigenesis.

Item Type: Article
Uncontrolled Keywords: Linico-Pathological Parameters; Mir-31; Mir-375; Microrna Profiling; Oral Squamous Cell Carcinoma
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral & Maxillofacial Surgery
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 14 Oct 2014 03:00
Last Modified: 21 Dec 2014 08:53
URI: http://eprints.um.edu.my/id/eprint/10479

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