Optimization of extraction parameters by using response surface methodology, purification, and identification of anthocyanin pigments in melastoma malabathricum fruit

Anuar, Nordiyanah and Mohd Adnan, Ahmad Faris and Saat, Naziz and Aziz, Norkasmani and Taha, Rosna Mat (2013) Optimization of extraction parameters by using response surface methodology, purification, and identification of anthocyanin pigments in melastoma malabathricum fruit. Scientific World Journal, 2013. p. 810547. ISSN 2356-6140, DOI https://doi.org/10.1155/2013/810547.

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Official URL: http://dx.doi.org/10.1155/2013/810547

Abstract

Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell. Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that can be recovered from Melastoma malabathricum fruit. Factors such as extraction temperature, extraction time, and solid to liquid ratio were identified to be significantly affecting anthocyanin extraction efficiency. By using three-level three-factor Box-Behnken design, the optimized conditions for anthocyanin extraction by acidified methanol (R (2) = 0.972) were temperature of 60°C, time of 86.82 min, and 0.5 : 35 (g/mL) solid to liquid ratio while the optimum extraction conditions by acidified ethanol (R (2) = 0.954) were temperature of 60°C, time of 120 min, and 0.5 : 23.06 (g/mL) solid to liquid ratio. The crude anthocyanin extract was further purified by using Amberlite XAD-7 and Sephadex LH-20 column chromatography. Identification of anthocyanins revealed the presence of cyanidin dihexoside, cyanidin hexoside, and delphinidin hexoside as the main anthocyanins in M. malabathricum fruit.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Optimization; Extraction Parameters; thocyanin Pigments in Anthocyanin Pigments; Melastoma malabathricum Fruit
Subjects: Q Science > Q Science (General)
Q Science > QC Physics
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Norhamizah Tamizi
Date Deposited: 17 Apr 2014 02:38
Last Modified: 09 Feb 2021 06:36
URI: http://eprints.um.edu.my/id/eprint/9620

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