Molecular characterization of Giardia duodenalis in Yemen

Alyousefi, N.A. and Mahdy, M.A.K. and Xiao, L.H. and Mahmud, R. and Lim, Y.A.L. (2013) Molecular characterization of Giardia duodenalis in Yemen. Experimental Parasitology, 134 (2). pp. 141-147. ISSN 0014-4894, DOI https://doi.org/10.1016/j.exppara.2013.03.001.

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Abstract

Giardia duodenalis is an important intestinal protozoan in Yemen with infection rates ranging from 18 to 27. To date, there has been no genotyping study to provide a better understanding of the transmission dynamic. This study was conducted to genotype and subtype G. duodenalis in Yemen. Stool samples were collected from 503 Yemeni outpatients between I and 80 years old, including 219 males and 284 females. Giardia cysts were detected via microscopy after the formal-ether concentration. Genotyping of Giardia was carried out using PCR and sequence analysis of the 16s rRNA and b-giardin genes. Of the 89 microscopy-positive Giardia samples, 65 were successfully sequenced, of which 66 (43 of 65) were identified as G. duodenalis assemblage A and 34 (22 of 65) as assemblage B. Further subtyping analysis based on b-giardin gene identified the presence of subtypes A2 and A3, which belong to the anthroponotic sub-assemblage All. Data of the study suggest that anthroponotic transmission played a potential role in the transmission of giardiasis in the community. However, further genotyping and subtyping studies of specimens from humans and animals living in the same households are needed for a more definitive understanding of giardiasis transmission in Yemen. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Alyousefi, Naela A. Mahdy, Mohammed A. K. Xiao, Lihua Mahmud, Rohela Lim, Yvonne A. L.
Uncontrolled Keywords:	Giardia duodenalis; Genotyping; Yemen
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms Haslinda Lahuddin
Date Deposited:	16 Dec 2013 03:59
Last Modified:	16 Dec 2013 03:59
URI:	http://eprints.um.edu.my/id/eprint/8696

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