Hiong, L.C. and Voon, K.L. and Abdullah, N.A. and Sattar, M.A. and Rahman, N.A. and Khan, A.H. and Johns, E.J. (2008) Effect of TGF-beta 1 antisense oligodeoxynucleotide on renal function in chronic renal failure rats. Acta Pharmacologica Sinica, 29 (4). pp. 451-457. ISSN 1671-4083, DOI https://doi.org/10.1111/j.1745-7254.2008.00772.x.
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Abstract
Aim: The aim of the present study was to investigate the effectiveness of transforming growth factor (TGF)-beta 1 antisense oligodeoxynucleotides (ODN) in ameliorating deteriorated kidney function in rats with puromycin-induced chronic renal failure (CRF). Methods: Saline, puromycin, puromycin+TGF-beta 1 antisense ODN or puromycin+scrambled ODN were administered to unilaterally nephrectomized rats. Renal hemodynamic and excretory measurements were taken in the anaesthetized rats that had undergone surgical procedure. Results: It was observed that in the CRF rats, there was a marked reduction in the renal blood flow (RBF), glomerular filtration rate (GFR), severe proteinuria, and almost 6-fold increased fractional excretion of sodium (FE Na+) as compared to that in the control rats (all P < 0.05). It was further observed that in the CRF rats, the treatment with TGF-beta 1 antisense, but not scrambled ODN, markedly attenuated the reduction of RBF, GFR, and proteinuria and markedly prevented the increase of the FE Na+ (all P < 0.05). In addition, the renal hypertrophy in the CRF group (P < 0.05 vs non-renal failure control) was markedly attenuated after treatment with TGF-1 antisense ODN (P < 0.05). Focal segmental glomerulosclerosis was evident only in the untreated and scrambled ODN-treated CRF groups. An interesting observation of this study was that in the CRF rats, although there was marked attenuating and preventive effects of the TGF-beta 1 antisense ODN on the deteriorated renal functions, the antisense treatment did not cause any marked change in the renal expression of TGF-beta 1 at the protein level. Conclusion: Collectively, the data obtained suggests that TGF-beta 1 antisense ODN possesses beneficial effects in puromycin-induced chronic renal failure and that the deterioration in morphology and impaired renal function in this pathological state is in part dependent upon the action of TGF-beta 1 within the kidney.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | |
| Uncontrolled Keywords: | Chronic renal failure, transforming growth factor-β1, antisense oligodeoxynucleotides, renal function |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Haslinda Lahuddin |
| Date Deposited: | 11 Nov 2013 03:36 |
| Last Modified: | 11 Nov 2013 03:36 |
| URI: | http://eprints.um.edu.my/id/eprint/8526 |
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