Abdullah, Ramli and Rahman, M.M. and Idris, S.K. and Wan Embong, W.K. (2013) Comparison between different combinations of chemical treatment on parthenogenetic activation of mouse oocytes and its subsequent embryonic development. Animal Cells and Systems, 17 (3). pp. 196-202. ISSN 19768354 , DOI https://doi.org/10.1080/19768354.2013.807877.
Full text not available from this repository.Abstract
Combination of different chemicals has been an effective method to activate oocytes. The objectives of this study were to evaluate the optimal: (1) concentration (Experiment 1) and duration (Experiment 2) of strontium chloride (SrCl2) in the presence of cytochalasin B (CB), (2) secondary activation agent [6-dimethylaminopurine (6-DMAP), cycloheximide (CHX) and CB] in combination with calcium ionophore (CaI) (Experiment 3) and (3) ethanol (EtOH) concentration in combination with 6-DMAP (Experiment 4). There were no significant (P>0.05) differences in cleavage (86.82-89.06%) and blastocyst (33.42-46.83%) rates when oocytes were treated with various concentrations of SrCl2 (2-10 mM). Three-hour duration generally showed the highest cleavage (93.83%) and blastocyst rates (60.42%) compared to other durations. Comparing with various combinations, CaI +6-DMAP showed the highest cleavage at all stages of development and significantly (P<0.05) higher from other combinations at 8-cell (50.58%), morula (30.29%) and blastocyst (23.28%) stages. When comparing the EtOH concentrations, 8% EtOH gave significantly (P<0.05) highest cleavage rates at 8-cell stage (39.70%), morula (21.50%) and blastocyst (13.86%) stages followed by 9% and 7% EtOH. In conclusion, 10 mM SrCl2+5 μg/ml CB in calcium-free Chatot Ziomek Bavister medium for 3-h incubation duration gave the highest cleavage rates of parthenotes mouse oocytes. © 2013 Copyright Korean Society for Integrative Biology.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
| Uncontrolled Keywords: | Embryonic development, Mouse oocytes, Parthenogenesis, Strontium chloride |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Miss Malisa Diana |
| Date Deposited: | 10 Oct 2013 01:21 |
| Last Modified: | 28 Apr 2021 08:09 |
| URI: | http://eprints.um.edu.my/id/eprint/8434 |
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