The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells

Awang, Khalijah and Azmi, Mohamad Nurul and Aun, Lionel In Lian and Aziz, Ahmad Nazif and Ibrahim, Halijah and Nagoor, Noor Hasima (2010) The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells. Molecules, 15 (11). pp. 8048-8059. ISSN 1420-3049, DOI https://doi.org/10.3390/molecules15118048.

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Abstract

1'-(S)-1'-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethnomedicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time-and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 mu M concentration. Flow cytometric analysis for annexin-V and PI dual staining demonstrated that cell death occurred via apoptosis, followed by secondary necrosis. The apoptotic effects of ACA were confirmed via the DNA fragmentation assay, in which consistent laddering of genomic DNA was observed for all tumour cell lines after a 24 hrs post-treatment period at the IC(50) concentration of ACA. A cell cycle analysis using PI staining also demonstrated that ACA induced cell cycle arrest at the G(0)/G(1) phase, corresponding to oral tumour cell lines. In conclusion, ACA exhibits enormous potential for future development as a chemotherapeutic drug against various malignancies.

Item Type:	Article
Funders:	UNSPECIFIED
Uncontrolled Keywords:	1’-(S)-1’-acetoxychavicol acetate; Alpinia conchigera; apoptosis; cytotoxicity; cell cycle arrest
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Institute of Biological Sciences Faculty of Science > Department of Chemistry
Depositing User:	miss munirah saadom
Date Deposited:	15 Jul 2013 23:37
Last Modified:	28 Aug 2019 06:39
URI:	http://eprints.um.edu.my/id/eprint/7785

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