Role of Nε-(Carboxymethyl)Lysine in the Development of Ischemic Heart Disease in Type 2 Diabetes Mellitus

Ahmed, Khaled A. and Muniandy, Sekaran and Ismail, Ikram Shah (2007) Role of Nε-(Carboxymethyl)Lysine in the Development of Ischemic Heart Disease in Type 2 Diabetes Mellitus. Journal of Clinical Biochemistry and Nutrition, 41 (2). pp. 97-105. ISSN 0912-0009, DOI https://doi.org/10.3164/jcbn.2007014.

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Abstract

This study aims to determine the levels of Nε-(carboxymethyl)lysine (CML) in patients with Type 2 diabetic patients with and without ischemic heart disease (IHD) and to find for a possible association between circulating CML and a number of clinical parameters including lipids, hemoglobin A1c (HbA1c) and malondialdehyde (MDA) in Type 2 diabetic IHD patients. Serum CML levels were measured by enzyme-linked immunosorbent assay using polyclonal anti-CML antibodies. Serum levels of CML and MDA were assessed in 60 IHD patients with Type 2 diabetes, 43 IHD patients without Type 2 diabetes, 64 Type 2 diabetics without IHD, and 80 sex- and age-matched healthy subjects. Correlations studies between CML levels and lipids, HbA1c, and lipid peroxidation were performed in Type 2 diabetes patients with and without IHD. A statistical significance was observed in the levels of serum glucose, lipids (triglyceride, total cholesterol, HDL-cholesterol), MDA, HbA1c, CML and LDL-cholesterol (p<0.05) between the groups of the study. CML levels were significantly increased in diabetic IHD patients compared with Type 2 diabetes patients but without IHD (537.1 ± 86.1 vs 449.7 ± 54.9, p<0.001). A positive correlation was observed between serum levels of CML and MDA, r = 0.338 (p = 0.008) in Type 2 diabetes patients with IHD. However, age, HbA1c and lipids had no significant influence on CML levels among diabetics (p>0.05). In conclusion, this study demonstrates the effect of both diabetes and oxidative stress on the higher levels of circulating CML. These results showed that increased serum levels of CML are associated with the development of IHD in Type 2 diabetes mellitus.

Item Type:	Article
Funders:	Intensive Research in Priorities Area (IRPA), Ministry of Science and Technology, Malaysia. (Project grant IRPA No. 36-02-03-6020).
Additional Information:	Times Cited: 10 Ahmed, Khaled A. Muniandy, Sekaran Ismail, Ikram S.
Uncontrolled Keywords:	advanced glycation end products; Nε-(carboxymethyl)lysine; Type 2 diabetes mellitus; ischemic heart disease; oxidative stress
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms Haslinda Lahuddin
Date Deposited:	12 Jul 2013 01:23
Last Modified:	10 Aug 2020 07:57
URI:	http://eprints.um.edu.my/id/eprint/7223

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