Baba, A.S.H. and Buttery, P.J. and Harper, J.M.M. (2000) Effects of gastric inhibitory polypeptide, somatostatin and epidermal growth factor on lipogenesis in ovine adipose explants. Comparative Biochemistry and Physiology Part B : Biochemistry & Molecular Biology, 127 (2). pp. 173-182. ISSN 0305-0491, DOI https://doi.org/10.1016/s0305-0491(00)00249-2.
Full text not available from this repository.Abstract
Feeding raises the plasma concentrations of a number of gut-related hormones that may, in turn, influence the metabolism of peripheral tissues. This study investigated the effects of gut-related hormones on lipogenesis in explants from three differing adipose depots in lambs (aged 4-9 months). Incorporation of [C-14]-acetate into lipid was measured over a 2-h period, following 24 h pre-incubation in the presence of hormone combinations. In perirenal fat explants, gastric inhibitory polypeptide (GTP) in the concentration range 0.01-10 nM stimulated lipogenesis. Maximal effects were seen at 1 nM (an average increase of 64% over basal values). In contrast, in the presence of insulin (0.1 nM), a dose-dependant decrease in lipogenesis was seen with increasing GIP concentration (P < 0.001 for the insulin x GIP interaction). Epidermal growth factor (EGF) and somatostatin in the same concentration range each inhibited lipogenesis, both in the presence and the absence of insulin (P < 0.001 in each case). Subcutaneous (back) fat and intermuscular (popliteal) fat responded similarly to each other, but significantly differently from the perirenal depot (P < 0.001). Here GIP, somatostatin or EGF (each at 1 nM) all separately stimulated lipogenesis. (C) 2000 Elsevier Science Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
| Uncontrolled Keywords: | Sheep; Adipose tissue; Depot fat; Lipid metabolism; Gastrointestinal hormones; Glucose-dependant insulinotropic polypeptide; Insulin; Stimulation; Inhibition; Interactions |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Miss Malisa Diana |
| Date Deposited: | 03 Jul 2013 03:39 |
| Last Modified: | 11 Dec 2013 02:26 |
| URI: | http://eprints.um.edu.my/id/eprint/6572 |
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