Tan, Eng Chong and Karsani, Saiful Anuar and Foo, Gen Teck and Wong, Sher Ming and Abd Rahman, Noorsaadah and Khalid, Norzulaani and Othman, Shatrah and Yusof, Rohana (2012) Proteomic analysis of cell suspension cultures of Boesenbergia rotunda induced by phenylalanine: identification of proteins involved in flavonoid and phenylpropanoid biosynthesis pathways. Plant Cell, Tissue and Organ Culture, 111 (2). pp. 219-229. ISSN 0167-6857, DOI https://doi.org/10.1007/s11240-012-0188-8.
Full text not available from this repository.Abstract
Boesenbergia rotunda belongs to the Zingiberaceae family. It is widely found throughout Southeast Asia and is commonly used as a food ingredient and in folk medicine. Extracts from this plant contain a number of important bioactive compounds such as boesenbergin, cardamonin, pinostrobin, pinocembrin, panduratin A and 4-hydroxypanduratin A. These compounds have been shown to exhibit anti-HIV protease, anti-dengue NS2B/ NS3 protease, antibacterial, antifungal, anti-inflammatory, anticancer, and antioxidant activity. Here we report the use of proteomic approaches to identify proteins that may be involved in the biosynthesis of these compounds. Protein expressions of B. rotunda suspension cultures for phenylalanine-treated and normal callus were compared by two-dimensional gel electrophoresis. Following image analysis, protein spots whose expressions were found to be regulated were identified using Matrix Assisted Laser Desorption-Ionization tandem mass spectrometry. In all, thirty four proteins were identified. These proteins were categorized into nine functional categories—defence mechanism, protein biosynthesis, metabolism, terpenoid biosynthesis, cell division, cell organization, energy-related, signaling processes and proteins of unknown function. Eleven of the proteins involved in the phenylpropanoid biosynthetic pathway are related to the biosynthesis of cyclohexenyl chalcone derivatives.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Boesenbergia rotunda; Flavonoids; Panduratin A; Proteomics; 4-Hydroxypanduratin A |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences Faculty of Medicine Faculty of Science > Department of Chemistry |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 07 May 2013 02:02 |
| Last Modified: | 22 Nov 2019 03:47 |
| URI: | http://eprints.um.edu.my/id/eprint/6005 |
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