Jalil, M. and Khalid, N. and Othman, R.Y. (2003) Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA). Plant Cell, Tissue and Organ Culture, 75 (3). pp. 209-214. ISSN 0167-6857, DOI https://doi.org/10.1023/A:1025814922547.
Full text not available from this repository.Abstract
Embryogenic callus was established using immature male flower of Musa acuminata cv. Mas. After 5-6 months of culture, embryogenic callus was obtained at 21.75±11.9 from 750 immature male flower clusters with translucent somatic embryos proliferated from the whitish friable callus. It was observed that flower clusters ranging from 4 to 11 responded to form embryogenic callus and out of which 3-10 somatic embryos were formed per flower cluster. Embryogenic callus were obtained at a percentage of 10.00±0.3 on M1 medium initially supplemented with 18 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for 3 months and subsequently transferred to the same media with reduced 2,4-D (9 μM) for the next 2-3 months. Embryos developed into translucent spheres and slightly torpedo shaped embryos in suspension cultures. Plantlets were obtained on medium M4 supplemented with 0.8 μM BA, at an average regeneration rate of 13.00±0.58.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Banana; Cell suspensions; Male inflorescence; Somatic embryos; Tissue culture |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 08 May 2013 01:05 |
| Last Modified: | 14 Jan 2019 01:58 |
| URI: | http://eprints.um.edu.my/id/eprint/6002 |
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