Efficient propagation of an important medicinal plant boesenbergia rotunda by shoot derived callus

Yusuf, N.A. and Annuar, M.S.M. and Khalid, N. (2011) Efficient propagation of an important medicinal plant boesenbergia rotunda by shoot derived callus. Journal of Medicinal Plants Research, 5 (13). pp. 2629-2636. ISSN 1996-0875,

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Abstract

A protocol for high frequency shoot organogenesis and plant establishment from shoot base derived callus was developed for Boesenbergia rotunda (L.) Mansf. Kulturpfl. The cultures produced callus in MS medium supplemented with various concentrations of dicamba and 2,4-D. Nevertheless, embryogenic callus only emerged on 2, 4-D augmented MS medium. In addition, the success of callus induction was achieved from meristem derived callus producing 100 of embryogenic callus. Multiple shoot induction was achieved from the surface of the callus after transferring onto MS medium supplemented with BAP or kinetin (shoot induction medium). The highest frequency of callus producing shoots (49.8) was achieved on MS medium containing 2.0 mg/L BAP. Plantlets with welldeveloped shoots and roots on MS medium supplemented with 2 g/L activated charcoal were acclimatized and successfully grown in the greenhouse.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Boesenbergia rotunda Medicinal plant Micropropagation Organogenesis Plant cell cultures Zingiberaceae 2,4 dichlorophenoxyacetic acid 6 n benzyladenine dicamba kinetin article callus (plant) concentration (parameters) controlled study greenhouse Murashige and Skoog medium nonhuman plant development plant growth plant root plantlet process development shoot
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 25 Apr 2013 01:27
Last Modified: 25 Apr 2013 01:27
URI: http://eprints.um.edu.my/id/eprint/5943

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