Direct regeneration and rapd assessment of male inflorescence derived plants of musa cuminata cv. berangan

Harirah, A.A. and Khalid, Norzulaani (2006) Direct regeneration and rapd assessment of male inflorescence derived plants of musa cuminata cv. berangan. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 11-17. ISSN 0128-7451,

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Abstract

Regenerated plants were established using male inflorescence of Musa acuminata cv. Berangan. Explants were cultured on Murashige and Skoog (MS) solid medium supplemented with three different concentrations of 6-benzylaminopurine (BAP). After 2 weeks, whitish bud-like structures (WBLS) were obtained and MS media supplemented with 70.0 μM BAP was observed as the best media for the growth of WBLS. After 3 months, shoot-like structures emerged and MS media supplemented with 31.0 μM BAP gave a large number of shoot formation. Normal looking plantlets were obtained within 4 - 6 months with an average of 80 - 130 shoots regenerated from each male inflorescence. Random amplified polymorphic DNA (RAPD) was carried out to determine the clonal fidelity on in vitro Musa acuminata cv. Berangan micropropagated from male inflorescence derived from the same mother plant. Eighteen arbitrary decamer primers were used to amplify DNA from in vitro plants materials. All RAPD profiles from regenerated plants were monomorphic thus no somaclonal variation was detected. A total of 81 bands were scored from PCR amplification of genomic DNA from 15 micropropagated plants. This was further confirmed by the value of similarity index which was equaled to 1. This result implied that male inflorescence could be used as an alternative explant for commercial planting.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Banana clonal propagation in vitro male inflorescence RAPD similarity index Musa Musa acuminata
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 24 Apr 2013 03:21
Last Modified: 11 Feb 2019 07:43
URI: http://eprints.um.edu.my/id/eprint/5925

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