Tee, Kok Keng and Chan, Po Qhuan and Loh, Alson Mun-Khin and Singh, Sarbhan and Teo, Chee How and Iyadorai, Thevambiga and Chook, Jack Bee and Ng, Kim Tien and Takebe, Yutaka and Chan, Kok Gan and Sam, I-Ching and Voon, Kenny (2023) Surveillance, isolation and genomic characterization of Pteropine orthoreovirus of probable bat origin among patients with acute respiratory infection in Malaysia. Journal of Medical Virology, 95 (2). ISSN 0146-6615, DOI https://doi.org/10.1002/jmv.28520.
Full text not available from this repository.Abstract
Pteropine orthoreovirus (PRV), an emerging bat-borne virus, has been linked to cases of acute respiratory infections (ARI) in humans. The prevalence, epidemiology and genomic diversity of PRV among ARI of unknown origin were studied. Among 632 urban outpatients tested negative for all known respiratory viruses, 2.2% were PRV-positive. Patients mainly presented with moderate to severe forms of cough, sore throat and muscle ache, but rarely with fever. Phylogenetic analysis revealed that over 90% of patients infected with the Melaka virus (MelV)-like PRV, while one patient infected with the Pulau virus previously found only in fruit bats. Human oral keratinocytes and nasopharyngeal epithelial cells were susceptible to clinical isolates of PRV, including the newly isolated MelV-like 12MYKLU1034. Whole genome sequence of 12MYKLU1034 using Nanopore technique revealed a novel reassortant strain. Evolutionary analysis of the global PRV strains suggests the continuous evolution of PRV through genetic reassortment among PRV strains circulating in human, bats and non-human primate hosts, creating a spectrum of reassortant lineages with complex evolutionary characteristics. In summary, the role of PRV as a common etiologic agent of ARI is evident. Continuous monitoring of PRV prevalence, pathogenicity and diversity among human and animal hosts is important to trace the emergence of novel reassortants.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia |
| Uncontrolled Keywords: | acute respiratory infection; fruit bats; Melaka virus; Nanopore sequencing; Pteropine orthoreovirus (PRV); Pulau virus; reassortment; zoonotic virus |
| Subjects: | Q Science > QH Natural history R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Medical Microbiology Department Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Jul 2025 05:06 |
| Last Modified: | 17 Jul 2025 05:06 |
| URI: | http://eprints.um.edu.my/id/eprint/50912 |
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