Untargeted lipidomics approach using LC-Orbitrap HRMS to discriminate lard from beef tallow and chicken fat for the authentification of halal

Windarsih, A. and Bakar, N. K. A. and Rohman, A. and Riswanto, F. D. O. and Erwantof, Y. (2023) Untargeted lipidomics approach using LC-Orbitrap HRMS to discriminate lard from beef tallow and chicken fat for the authentification of halal. Grasas y Aceites, 74 (3). ISSN 0017-3495, DOI https://doi.org/10.3989/gya.0980221.

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Abstract

This research aimed to perform a lipidomics study using liquid chromatography-high resolution mass spectrometry (LCHRMS) to identify lard, beef tallow and chicken fat. A total of 292, 345, and 403 lipid compounds were observed in lard, beef tallow, and chicken fat, respectively. The lipid groups of AcHexStE (acyl hexosyl stigmasterol ester), biotinylPE (biotinylphosphoetanolamine), LPC (lysophosphatidylcholine), MePC (monoetherphosphatidylcholine), PC (phosphatidylcholine) and PI (phosphoinocitol) were found to be specific for lard. The principal component analysis ( PCA) and partial least square-discriminant analysis (PLS-DA) successfully differentiated lard from beef tallow and chicken fat. This research suggested that the untargeted lipidomics technique using LC-HRMS combined with chemometrics could be used to discriminate lard from beef tallow and chicken fat. This method is a promising technique for the detection of lard adulteration in beef tallow and chicken fat for halal authentication purposes.

Item Type: Article
Funders: Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) (1817/UN1/DITLIT/Dit-Lit/PT.01.03/2022), LPDP (Lembaga Pengelola Dana Pendidikan) Scholarship (Indonesian Endowment Fund for Education), Ministry of Finance, Republic of Indonesia
Uncontrolled Keywords: Chemometrics; Halal Authentication; Lard; LC-HRMS; Metabolomics; Untargeted
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 07 Aug 2025 06:46
Last Modified: 07 Aug 2025 06:46
URI: http://eprints.um.edu.my/id/eprint/50735

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