Kumar, Gowri Gopa and Kilari, Eswar Kumar and Nelli, Giribabu and Salleh, Naguib (2023) Oral administration of Turnera diffusa willd. ex Schult. extract ameliorates steroidogenesis and spermatogenesis impairment in the testes of rats with type-2 diabetes mellitus. Journal Of Ethnopharmacology, 314. ISSN 0378-8741, DOI https://doi.org/10.1016/j.jep.2023.116638.
Full text not available from this repository.Abstract
Ethnopharmacological relevance: Turnera diffusa Willd. ex Schult. (T. diffusa) has traditionally been used to treat male reproductive dysfunction and have aphrodisiac properties.Aims of the study: This study aims to investigate the ability of T. diffusa to ameliorate the impairment in testicular steroidogenesis and spermatogenesis in DM that might help to improve testicular function, and sub-sequently restore male fertility.Materials and methods: DM-induced adult male rats were given 100 mg/kg/day and 200 mg/kg/day T. diffusa leaf extract orally for 28 consecutive days. Rats were then sacrificed; sperm and testes were harvested and sperm parameter analysis were performed. Histo-morphological changes in the testes were observed. Biochemical as-says were performed to measure testosterone and testicular oxidative stress levels. Immunohistochemistry and double immunofluorescence were used to monitor oxidative stress and inflammation levels in testes as well as Sertoli and steroidogenic marker proteins' expression.Results: Treatment with T. diffusa restores sperm count, motility, and viability near normal and reduces sperm morphological abnormalities and sperm DNA fragmentation in diabetic rats. T. diffusa treatment also reduces testicular NOX-2 and lipid peroxidation levels, increases testicular antioxidant enzymes (SOD, CAT, and GPx) activities, ameliorates testicular inflammation via downregulating NF-Kappa B, p-Ikk beta and TNF-alpha and upregulating I kappa B alpha expression. In diabetic rats, T. diffusa treatment increases testicular steroidogenic proteins (StAR, CYP11A1, SHBG, and ARA54, 3 and 17 beta-HSD) and plasma testosterone levels. Furthermore, in diabetic rats treated with T. diffusa, Sertoli cell marker proteins including Connexin 43, N-cadherin, and occludin levels in the testes were elevated.Conclusion: T. diffusa treatment could help to ameliorate the detrimental effects of DM on the testes, thus this plant has potential to be used to restore male fertility.
| Item Type: | Article |
|---|---|
| Funders: | AICTE RPS project , India [Grant No: A.V. (3) /AICTE-RPS/2014] |
| Uncontrolled Keywords: | DM-Induced male infertility; T; diffusa; Testes; Oxidative stress; Inflammation; Steroidogenesis; Spermatogenesis |
| Subjects: | Q Science > QD Chemistry R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine > Physiology Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 06 Nov 2025 03:30 |
| Last Modified: | 06 Nov 2025 03:30 |
| URI: | http://eprints.um.edu.my/id/eprint/49790 |
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