Subermaniam, Kogilavani and Lew, Sze Yuen and Yow, Yoon Yen and Lim, Siew Huah and Yu, Wing Shan and Lim, Lee Wei and Wong, Kah Hui (2023) Malaysian brown macroalga Padina australis mitigates lipopolysaccharide-stimulated neuroinflammation in BV2 microglial cells. Iranian Journal of Basic Medical Sciences, 26 (6). pp. 669-679. ISSN 2008-3866, DOI https://doi.org/10.22038/IJBMS.2023.67835.14842.
Full text not available from this repository.Abstract
Objective(s): Neuroinflammation and microglial activation are pathological features in central nervous system disorders. Excess levels of reactive oxygen species (ROS) and pro-inflammatory cytokines have been implicated in exacerbation of neuronal damage during chronic activation of microglial cells. Padina australis, a brown macroalga, has been demonstrated to have various pharmacological properties such as anti-neuroinflammatory activity. However, the underlying mechanism mediating the anti-neuroinflammatory potential of P. australis remains poorly understood. We explored the use of Malaysian P. australis in attenuating lipopolysaccharide (LPS)-stimulated neuroinflammation in BV2 microglial cells.Materials and Methods: Fresh specimens of P. australis were freeze-dried and subjected to ethanol extraction. The ethanol extract (PAEE) was evaluated for its protective effects against 1 mu g/ml LPS-stimulated neuroinflammation in BV2 microglial cells.Results: LPS reduced the viability of BV2 microglia cells and increased the levels of nitric oxide (NO), prostaglandin E2 (PGE2), intracellular reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6). However, the neuroinflammatory response was reversed by 0.5-2.0 mg/ml PAEE in a dose-dependent manner. Analysis of liquid chromatography-mass spectrometry (LC-MS) of PAEE subfractions revealed five compounds; methyl alpha-eleostearate, ethyl alpha-eleostearate, niacinamide, stearamide, and linoleic acid. Conclusion: The protective effects of PAEE against LPS-stimulated neuroinflammation in BV2 microglial cells were found to be mediated by the suppression of excess levels of intracellular ROS and pro-inflammatory mediators and cytokines, denoting the protective role of P. australis in combating continuous neuroinflammation. Our findings support the use of P. australis as a possible therapeutic for neuroinflammatory and neurodegenerative diseases.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Faculty of Medicine Research Grant [Grant No: GPF003C-2019], Sunway University Internal Grant [Grant No: INT-2019-SST-DBS-04], Public Service Department Malaysia, Ministry of Health Malaysia, Federal Training Prize (HLP) [Grant No: KKM500-7/92/730525085970 (LDP 1) (6)] |
| Uncontrolled Keywords: | Brown algae; BV2 microglial; Cytokines; Major compounds; Neuroinflammation; Oxidative damage |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 08 Nov 2025 03:28 |
| Last Modified: | 08 Nov 2025 03:28 |
| URI: | http://eprints.um.edu.my/id/eprint/49775 |
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