Kuan, Chee Sian and Ng, Kee Peng and Yew, Su Mei and Meleh, Hadiza Umar and Seow, Heng Fong and How, Kang Nien and Yeo, Siok Koon and Jee, Jap Meng and Tan, Yung-Chie and Yee, Wai-Yan and Hoh, Chee-Choong and Velayuthan, Rukumani Devi and Na, Shiang Ling and Masri, Siti Norbaya and Chew, Shu Yih and Than, Leslie Thian Lung (2023) Comparative genomic and phenotypic analyses of pathogenic fungi Neoscytalidium dimidiatum and Bipolaris papendorfii isolated from human skin scraping. Brazilian Journal of Microbiology, 54 (3). pp. 1351-1372. ISSN 1517-8382, DOI https://doi.org/10.1007/s42770-023-01032-z.
Full text not available from this repository.Abstract
Neoscytalidium dimidiatum and Bipolaris species are fungal plant pathogens that have been reported to cause human diseases. Recently, we have isolated numerous N. dimidiatum and Bipolaris species from the skin scrapings and nails of different patients. In this work, we have sequenced the genome of one strain of N. dimidiatum. The sequenced genome was compared to that of a previously reported Bipolaris papendorfii genome for a better understanding of their complex lifestyle and broad host-range pathogenicity. Both N. dimidiatum UM 880 (similar to 43 Mb) and B. papendorfii UM 226 (similar to 33 Mb) genomes include 11,015-12,320 putative coding DNA sequences, of which 0.51-2.49% are predicted transposable elements. Analysis of secondary metabolism gene clusters revealed several genes involved in melanin biosynthesis and iron uptake. The arsenal of CAZymes related to plants pathogenicity is comparable between the species, including genes involved in hemicellulose and pectin decomposition. Several important gene encoding keratinolytic peptidases were identified in N. dimidiatum and B. papendorfii, reflecting their potential pathogenic role in causing skin and nail infections. In this study, additional information on the metabolic features of these two species, such as nutritional profiling, pH tolerance, and osmotolerant, are revealed. The genomic characterization of N. dimidiatum and B. papendorfii provides the basis for the future functional studies to gain further insights as to what makes these fungi persist in plants and why they are pathogenic to humans.
| Item Type: | Article |
|---|---|
| Funders: | High Impact Research MOE Grant from the Ministry of Education Malaysia [Grant No: UM.C/625/1/HIR/MOHE/MED/31; H-20001 00-E000070], Putra Grant-Putra Graduate Initiative (IPS) from Universiti Putra Malaysia [Grant No: GP-IPS/2018/9613300] |
| Uncontrolled Keywords: | Dematiaceous; Fungal plant pathogen; Neoscytalidium dimidiatum; Bipolaris papendorfii; Skin scraping |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine > Medical Microbiology Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 30 Oct 2025 06:53 |
| Last Modified: | 30 Oct 2025 06:53 |
| URI: | http://eprints.um.edu.my/id/eprint/48719 |
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