Chen, Jzit Weii and Liew, Fong Fong and Tan, Hsiao Wei and Misran, Misni and Chung, Ivy (2023) Cholesterol-linoleic acid liposomes induced extracellular vesicles secretion from immortalized adipose-derived mesenchymal stem cells for in vitro cell migration. Artificial Cells Nanomedicine and Biotechnology, 51 (1). pp. 346-360. ISSN 2169-1401, DOI https://doi.org/10.1080/21691401.2023.2237534.
Full text not available from this repository.Abstract
Extracellular vesicles (EVs) are small vesicles that are naturally released by cells and play a crucial role in cell-to-cell communication, tissue repair and regeneration. As naturally secreted EVs are limited, liposomes with different physicochemical properties, such as 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and linoleic acid (LA) with modifications have been formulated to improve EVs secretion for in vitro wound healing. Various analyses, including dynamic light scattering (DLS) and transmission electron microscopy (TEM) were performed to monitor the successful preparation of different types of liposomes. The results showed that cholesterol-LA liposomes significantly improved the secretion of EVs from immortalized adipose-derived mesenchymal stem cells (AD-MSCs) by 1.5-fold. Based on the cell migration effects obtained from scratch assay, both LA liposomal-induced EVs and cholesterol-LA liposomal-induced EVs significantly enhanced the migration of human keratinocytes (HaCaT) cell line. These findings suggested that LA and cholesterol-LA liposomes that enhance EVs secretion are potentially useful and can be extended for various tissue regeneration applications.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme, Ministry of Higher Education, Malaysia [Grant No: FRGS/1/2019/SKK08/MAHSA/02/5] |
| Uncontrolled Keywords: | Cholesterol; Extracellular vesicles; Immortalized AD-MSCs; Liposomes; Regenerative medicine |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine Faculty of Science |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Nov 2025 07:06 |
| Last Modified: | 03 Nov 2025 07:06 |
| URI: | http://eprints.um.edu.my/id/eprint/48568 |
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