Tan, Suh Nih and Kotaki, Yuichi and Teng, Sing Tung and Lim, Hong Chang and Gao, Chunlei and Lundholm, Nina and Wolf, Matthias and Gu, Haifeng and Lim, Po Teen and Leaw, Chui Pin (2025) Intraspecific genetic diversity with unrestricted gene flow in the domoic acid-producing diatom Nitzschia navis-varingica (Bacillariophyceae) from the Western Pacific. Harmful Algae, 141. p. 102769. ISSN 1568-9883, DOI https://doi.org/10.1016/j.hal.2024.102769.
Full text not available from this repository.Abstract
The benthic pennate diatom Nitzschia navis-varingica, known for producing domoic acid (DA) and its isomers, is widely distributed in the Western Pacific (WP) region. To investigate the genetic differentiation and gene flow patterns among the populations in the WP, the genetic diversity of 354 strains of N. navis-varingica was analysed using two nuclear-encoded rDNA loci: the large subunit rDNA (LSU rDNA) and the internal transcribed spacer 2 (ITS2). Frustule morphology of each strain was examined by TEM. The LSU rDNA phylogeny revealed a monophyletic lineage encompassing all strains, with sequence divergences of <0.9 %. Phylogenetic and population genetic analyses of ITS2 identified eight distinct clades (designated as Groups A to H) with moderate to high genetic heterogeneity (0.5-19.7 %). The low genetic differentiations between the geographically separated populations (pairwise FST of <0.03) suggested high gene flow and lack of spatial genetic structuring. Molecular clock analysis of the ITS2 phylogeny traced the evolutionary history of N. navis-varingica to the Eocene Epoch, and the split between clades likely occurred from the mid-Miocene to Pleistocene Epochs (10.8-1.2 Ma). The population dispersal in the WP were likely influenced by historical events like the Quarternary glacial cycles during the period, contributing to its homogenous distributions in the region.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia, Higher Center of Excellence Fund [HICoE IOES-2023B] (LRGS/1/2020/UMT/01/1/3) |
| Uncontrolled Keywords: | Amnesic shellfish poisoning; Diatom; Frustule; Harmful algal bloom; Second internal transcribed spacer; Transmission electron microscopy |
| Subjects: | Q Science > QH Natural history > QH301 Biology S Agriculture > SH Aquaculture. Fisheries. Angling |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 16 Apr 2025 07:22 |
| Last Modified: | 16 Apr 2025 07:22 |
| URI: | http://eprints.um.edu.my/id/eprint/48062 |
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