Fukuda, Masako and Otsuka, Yasushi and Sakai, Kumiko and Uni, Shigehiko and Junker, Kerstin and Saeung, Atiporn and Srisuka, Wichai and Takaoka, Hiroyuki (2025) Metabolomic analysis of larval stages of Onchocerca japonica (Spirurida: Onchocercidae), raised in black fly (Diptera: Simuliidae) vectors, by gas chromatography-tandem mass spectrometry. Acta Tropica, 263. p. 107541. ISSN 0001-706X, DOI https://doi.org/10.1016/j.actatropica.2025.107541.
Full text not available from this repository.Abstract
To monitor and prevent the spread of zoonotic onchocerciasis, identification of the natural vectors (bloodsucking insects) of its causative agents, Onchocerca species, is crucial. To date, vector identification depends on the detection of infective third-stage larvae in insects by traditional dissection. We aimed to develop a novel, more efficient method for the discrimination of the four larval stages, i.e. microfilariae (Mf), first-stage larvae (L1), second-stage larvae (L2), and third-stage larvae (L3), of O. japonica by metabolomic analysis. Microfilariae of O. japonica, the causative agent of zoonotic onchocerciasis in Japan, were obtained from skin snips of wild boars and injected into newly-emerged black flies (Diptera: Simuliidae) to enable further larval development. Metabolites obtained from Mf, L1, L2, and L3 were analyzed using a gas chromatography-tandem mass spectrometer. Multivariate analysis of the data of metabolites showed the complete separation of the four larval stages. The highest level of acetoacetic acid and hydroxylamine was found in Mf and L3, respectively. Consequently, we propose that hydroxylamine is a potential marker to detect infective larvae of O. japonica in natural infections and could be a valuable tool in vector surveys, transmission studies and epidemiological surveys.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) (19K06421) |
| Uncontrolled Keywords: | Metabolomic analysis; Blood-sucking insects; Black fly; Onchocerca japonica; Third-stage larva; Zoonotic onchocerciasis |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 30 Apr 2025 06:17 |
| Last Modified: | 30 Apr 2025 06:17 |
| URI: | http://eprints.um.edu.my/id/eprint/47883 |
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