Alaidarous, Hassan Shaikh and Bhassu, Subha and Chandramathi, Samudi and Yusoff, Ismail and Soo, Tze Chiew Christie (2025) Genetic expression of retinol dehydrogenase 12 and all-trans-retinol 13,14-reductase during acute hepatopancreatic necrosis disease in Penaeus monodon: insight into retinoids metabolism in pathogenesis. Aquaculture International, 33 (3). ISSN 0967-6120, DOI https://doi.org/10.1007/s10499-025-01878-0.
Full text not available from this repository.Abstract
Vibrio parahaemolyticus (VpAHPND), the bacterium that causes acute hepatopancreatic necrosis disease (AHPND), is a severe hazard to shrimp aquaculture worldwide. Despite substantial research into AHPND pathophysiology, the role of retinoid metabolism in shrimp immunological responses remains unknown. We aimed to investigate the functions of retinol dehydrogenase 12 (RDH12) and all-trans-retinol 13,14-reductase (AT-R 13,14-R) during VpAHPND infection to better understand their contribution to retinoid metabolism and immunological responses in Penaeus monodon. Toward this, hepatopancreatic tissue samples were obtained at different time points (0, 3, 6, 12, 24, 36, and 48 h post-infection (hpi)) from P. monodon infected with VpAHPND (KS17.S5-1 strain). Quantitative real-time PCR (qRT-PCR) analysis of these samples revealed a substantial overexpression of RDH12 (sixfold) in the infected group compared to the control, with a peak expression of 6.11 +/- 0.15-fold at 12 hpi. AT-R 13,14-R showed a ninefold downregulation, with the lowest expression of - 9.69 +/- 0.04-fold at 3 hpi. These findings suggest that AHPND affects the retinoid signaling system, jeopardizing immune defenses and metabolic control. Thus, restoring this system may reduce the impact of harmful chemicals, leading to new treatment options, such as dietary supplementation with retinoid precursors, to protect shrimp farms against AHPND.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education - Kingdom of Saudi Arabia |
| Uncontrolled Keywords: | Penaeus monodon; Retinol dehydrogenase; Hepatopancreas; Vibrio parahaemolyticus; All-trans-retinol 13,14-reductase |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Oct 2025 08:01 |
| Last Modified: | 03 Oct 2025 08:01 |
| URI: | http://eprints.um.edu.my/id/eprint/47828 |
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