Nor, Sharifah Norsyahindah Syed and Rasanang, Nur Syafiqah and Karman, Salmah and Zaman, Wan Safwani Wan Kamarul and Harun, Sulaiman Wadi and Arof, Hamzah (2024) Incorporation of Thiol-Modified and Carboxyl-MoS2 Films as Support Layer in Surface Plasmon Resonance Sensing Platform for SARS-CoV-2 Spike Protein Detection. IEEE Sensors Journal, 24 (5). pp. 6026-6037. ISSN 1530-437X, DOI https://doi.org/10.1109/JSEN.2024.3349390.
Full text not available from this repository.Abstract
In the event of a severe acute respiratorysyndrome-coronavirus-2 (SARS-CoV-2) outbreak, rapid viraldetection is crucial for proper patient management. Althoughthe development of surface plasmon resonance (SPR)in optical biosensing for rapid viral detection is grow-ing, gold (Au) as a metallic sensing surface for SPRbiosensors exhibits inadequate sensitivity for sensitive viralrecognition. Hence, this study attempted to fabricate arapid and ultrasensitive SPR detection platform using dif-ferent support layers, such as a self-assembled mono-layer (SAM), consisting of 11-Mercaptoundecanoic acid(11-MUA), and 3-Mercaptopropionic acid (3-MPA) in addi-tion to transition-metal dichalcogenide (TMDC) carboxyl-molybdenum disulfide (MoS2-COOH) for the detection ofSARS-CoV-2 spike protein (S protein). The sensing surfacewas characterized using Raman spectroscopy, Fourier trans-form infrared (FTIR) spectroscopy, and transmission electronmicroscopy (TEM) before and after depositions, followed bySPR measurement testing with variation concentration of SARS-CoV-2 S protein. Performance parameters such assensitivity, full-width at half-maximum (FWHM), detection accuracy (DA), figure of merit (FoM), quality factor (QF), andlimit of detection (LoD) are calculated and compared between 11-MUA/Au/BK7 prism, 3-MPA/Au/BK7 prism, and MoS2-COOH/Au/BK7 prism in the detection of SARS-CoV-2 S protein. Results show the SPR setup with MoS2-COOH singlelayer exhibited the highest sensitivity (203.55 degrees/RIU) compared to 11-MUA (89.89(degrees)/RIU) and 3-MPA (108.90(degrees)/RIU). Overall,all three configurations demonstrate good linearity, excellent sensitivity, and LoD in the ng/mL range. The outcome ofthis study hoped to aid in the future development of a noninvasive, rapid, and ultrasensitive SPR detection platform forviral detection in minimal concentration.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education of Malaysia (MOHE) through the Fundamental Research Grant Scheme (FP114-2020) |
| Uncontrolled Keywords: | Biosensor; carboxyl molybdenum disulfide (MoS2-COOH); self-assembled monolayer (SAM); sensitivity enhancement; severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2); surface plasmon resonance (SPR) |
| Subjects: | R Medicine > R Medicine (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering |
| Divisions: | Faculty of Engineering > Biomedical Engineering Department Faculty of Engineering > Department of Electrical Engineering Faculty of Pharmacy > Department of Pharmaceutical Life Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 07 Jan 2025 08:15 |
| Last Modified: | 07 Jan 2025 08:15 |
| URI: | http://eprints.um.edu.my/id/eprint/47034 |
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