Tijani, Yahaya and Zanna, Hassan and Hock, Tan Choo and Shettima, Abubakar and Onu, Andrew and Sugun, Manasa and Ehizibolo, David and Shuaibu, Abdulmalik Bello and Habib, Abdulrazaq Garba (2024) Experimental production and efficacy testing of mono-specific antibodies against the venom of carpet viper (Echis ocellatus) from savannah Nigeria. Toxicon, 248. p. 107845. ISSN 0041-0101, DOI https://doi.org/10.1016/j.toxicon.2024.107845.
Full text not available from this repository.Abstract
Echis ocellatus is one of the commonest snakes responsible for envenomation in Nigeria. Antivenom is the only effective treatment, but the country suffers from a limited supply of effective antivenom. This study therefore aimed to explore the feasibility of effective, mono-specific antibodies production through immunization in rabbits using the venom of Echis ocellatus from Nigeria. The World Health Organization guide on antivenom production was employed in the immunization and the resultant antibodies were purified using protein A agarose column chromatography. Antibody titer reached a high plateau by 2-month immunization, and SDS PAGE of the sera suggests the presence of intact immunoglobulins accompanied with the heavy (50 kDa) and light (25 kDa) chains. The venom has an intravenous LD50 of 0.35 mg/kg in mice, and the venom lethality at a challenge dose of 2 LD50 was effectively neutralized by the antibodies with a potency value of 0.83 mg venom per g antibodies. The antibodies also neutralized the procoagulant activity of the venom with an effective dose (ED) of 13 +/- 0.66 mu l, supporting its use for hemotoxic envenomation. The study establishes the feasibility of developing effective, mono-specific antibodies against the Nigerian Carpet viper.
| Item Type: | Article |
|---|---|
| Funders: | Tertiary Education Trustfund (TETFUND) experimental grant, International Union of Biochemistry and Molecular Biology (IUMB) |
| Uncontrolled Keywords: | Snake venom; Echis ocellatus; Antibody production; Antivenom |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 27 Mar 2025 06:55 |
| Last Modified: | 27 Mar 2025 06:55 |
| URI: | http://eprints.um.edu.my/id/eprint/46781 |
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