Ong, Hui LinG and Tan, Choo Hock and Lee, Louisa Pernee and Khor, Sook Mei and Tan, Kae Yi (2022) An immunodetection assay developed using cobra cytotoxin-specific antibodies: Potential diagnostics for cobra envenoming. TOXICON, 216. pp. 157-168. ISSN 1879-3150, DOI https://doi.org/10.1016/j.toxicon.2022.07.010.
Full text not available from this repository.Abstract
Cobra (Naja spp.) envenoming is a life-threatening medical emergency, and a correct diagnosis is crucial to initiating timely and appropriate antivenom treatment. However, snakebite diagnostics remain unavailable in Southeast Asia. This study, therefore, developed an immunodetection assay with a potential diagnostic application for cobra envenoming. The cytotoxin of Naja kaouthia (Thai Monocled Cobra) (Nk-CTX) was purified from its venom to produce CTX-specific antibodies in rabbits and chickens. A double-antibody sandwich enzymelinked immunosorbent assay was developed using the purified anti-Nk-CTX antibodies (immunoglobulin G and immunoglobulin Y), and its selectivity, specificity, and sensitivity for the venoms of five major cobra species in Southeast Asia (N. kaouthia, Naja sumatrana, Naja sputatrix, Naja siamensis, and Naja philippinensis) were studied. The results showed the immunoassay discriminates cobra venoms from other species commonly implicated in snakebites in Southeast Asia, i.e., the Malayan Krait, Many-banded Krait, King Cobra, Eastern Russell's Viper, Malayan Pit Viper and White-lipped Pit Viper. The immunoassay has a high sensitivity for the five cobra venoms, with detection limits (LoD) ranging from 0.6 to 2.6 ng/ml. Together, the findings suggest the potential diagnostic application of the cytotoxin immunoassay for cobra envenoming. The immunoassay was found to exhibit high immunoreactivity toward ten Asiatic cobra venoms (absorbance > 1.5), in contrast to African cobra venoms with low immunoreactivity (absorbance < 0.9). Considering the varying CTX antigenicity between Asiatic and African cobras, the immunoassay for African cobras should utilize antibodies produced specifically from the cytotoxins of African cobra venoms.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya Specialist Centre CA.R.E research fund [PV040-2019], Universiti Malaya [GPF009C-2018] |
| Uncontrolled Keywords: | Snakebite envenoming; Cardiotoxins Immunodiagnostic assay; Toxin-specific polyclonal antibody; Double-antibody sandwich ELISA |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine > Department of Molecular Medicine Faculty of Science > Department of Chemistry |
| Depositing User: | Ms Koh Ai Peng |
| Date Deposited: | 23 Jul 2024 04:52 |
| Last Modified: | 23 Jul 2024 04:52 |
| URI: | http://eprints.um.edu.my/id/eprint/46259 |
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