Fauzi, Nurul Syazwani Ahmad and Abd Rahim, Muhamad Hafiz and Majid, Nazia Abdul and Othman, Rashidi and Yaacob, Jamilah Syafawati (2022) Evaluation of the effect of jasmonic acid elicitation on composition of pigments and biological activities in green callus of neem (Azadirachta indica). FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, 6. ISSN 2571-581X, DOI https://doi.org/10.3389/fsufs.2022.1017398.
Full text not available from this repository.Abstract
This study was carried out with the aim of determining the effects of jasmonic acid (JA) elicitation on the bioactive pigments' biosynthesis and the antioxidant activities in green callus of Azadirachta indica of two different ages (4- and 8-week-old). Plant tissue culture technique was employed to induce the formation of green callus from leaf explants of A. indica on Murashige and Skoog (MS) medium supplemented with 0.6 mg/L thidiazuron (CM) and three different concentrations of JA (2, 4, and 6 mg/L). The methanolic extracts from the green callus were used for determination of total chlorophyll content (TCh), total carotenoid content (TC), total anthocyanin content (TAC), total phenolic content (TPC), and total flavonoid content (TFC) through colorimetric and HPLC analyses. The highest amount of yield was obtained from CM and 2 mg/L JA (2JA) extracts for 4- and 8-week-old samples, respectively. Phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, and terpenoids in all 4- and 8-week-old samples elicited with 2, 4 and 6 mg/L JA. The highest value for TAC, TCh, TC, TPC, and TFC of 4- and 8-week-old samples were from callus cultured on media supplemented with 6 mg/L JA (6JA) and 4 mg/L JA (4JA), respectively. The lowest IC50 values were found to be 8.29 +/- 0.10 mg/mL (6JA) for 4-week-old and 7.73 +/- 0.03 mg/mL (4JA) for 8-week-old samples. The highest Ferric Reducing Antioxidant Power (FRAP) values obtained in this study were 90.60 +/- 1.55 g/g (6JA), and 74.59 +/- 3.91 g/g (4JA), respectively, for 4- and 8-week-old samples. Moreover, Pearson's correlation analysis revealed a significant correlation between TAC, TCh, TC, TPC, and TFC with ABTS and FRAP assays. In addition, PCA analysis revealed that 83.5% of the information (variances) contained in the data were retained by the first two principal components. Overall, these findings suggested that JA supplementation into the culture media significantly increase the chlorophyll, carotenoid, anthocyanin, phenolic and flavonoid contents and JA concentrations at 6 mg/L JA and 4 mg/L JA yielded the highest pigments content in 4- and 8-weeks-old callus, respectively.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya, Malaysia, Ministry of Education, Malaysia '[ST009-2021], Ministry of Education, Malaysia [FP055-2017A], Ministry of Education, Malaysia [FRGS/1/2017/WAB01/UM/02/2], Ministry of Education, Malaysia [01-01-20-2323FR], Ministry of Education, Malaysia [FRGS/1/2020/STG01/UPM/02/2] |
| Uncontrolled Keywords: | jasmonic acid; Azadirachta indica; plant tissue culture; callus age; bioactive pigments; antioxidant activities |
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms Koh Ai Peng |
| Date Deposited: | 29 Oct 2024 06:12 |
| Last Modified: | 29 Oct 2024 06:12 |
| URI: | http://eprints.um.edu.my/id/eprint/46156 |
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