Yusop, Mohd Hazim Mohd and Bakar, Mohd Fadzelly Abu and Kamarudin, Kamarul Rahim and Mokhtar, Nur Fadhilah Khairil and Hossain, Mohd Abd Motalib and Johan, Mohd Rafie and Noor, Nor Qhairul Izzreen Mohd (2022) Rapid detection of porcine DNA in meatball using recombinase polymerase amplification couple with lateral flow immunoassay for halal authentication. MOLECULES, 27 (23). ISSN 1420-3049, DOI https://doi.org/10.3390/molecules27238122.
Full text not available from this repository.Abstract
Point-of-care diagnostic methods for animal species determination are critical for rapid, simple, and accurate enforcement of food labelling. PCR is the most common method for species identification. However, the requirement of using a thermal cycler created drawbacks for the PCR application, particularly in low-resource settings. Hence, in this study, a method for porcine DNA detection using recombinase polymerase amplification (RPA), coupled with nucleic acid lateral flow immunoassay (NALFIA), was developed. Porcine-specific primers targeting pig (Sus scrofa) cytochrome b gene fragments specifically amplify a 197 bp fragment of the mitochondrial gene as being visualized by 2% agarose gel and PCRD NALFIA. The reaction temperature and time were 39 degrees C and 20 min, respectively. Herein, the specificity of the primers to porcine was confirmed after being assayed against six animal species, namely cow, goat, chicken, duck, dog, and rabbit. The porcine-specific RPA assay shows a high limit of detection of 0.01 ng/mu L pork DNA. Based on the preliminary performance data obtained from this study, the potential of this method as a rapid and sensitive tool for porcine DNA detection in meat-based products is foreseen.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | porcine DNA; RPA; NALFIA; meatball; halal authentication |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Nanotechnology & Catalysis Research Centre |
| Depositing User: | Ms Koh Ai Peng |
| Date Deposited: | 29 Oct 2024 06:17 |
| Last Modified: | 29 Oct 2024 06:17 |
| URI: | http://eprints.um.edu.my/id/eprint/46148 |
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