Azlan, U. W. and Lau, Yee Ling and Hamid, M. H. A. and Jelip, J. and Ooi, C. H. and Mudin, R. N. and Jaimin, J. J. and Fong, M. Y. (2022) Genetic diversity of secreted protein with an altered thrombospondin repeat (SPATR) of Plasmodium knowlesi clinical isolates from Malaysia. TROPICAL BIOMEDICINE, 39 (4). pp. 504-510. ISSN 0127-5720, DOI https://doi.org/10.47665/tb.39.4.006.
Full text not available from this repository.Abstract
The Plasmodium knowlesi secreted protein with an altered thrombospondin repeat (PkSPATR) is an important protein that helps in the parasite's invasion into the host cell. This protein has been regarded as one of the potential vaccine candidates against P. knowlesi infection. This study investigates the genetic diversity and natural selection of PkSPATR gene of P. knowlesi clinical isolates from Malaysia. PCR amplification of the full length PkSPATR gene was performed on 60 blood samples of infected P. knowlesi patients from Peninsular Malaysia and Malaysian Borneo. The amplified PCR products were cloned and sequenced. Sequence analysis of PkSPATR from Malaysia showed higher nucleotide diversity (CDS p: 0.01462) than previously reported Plasmodium vivax PvSPATR (p = 0.0003). PkSPATR from Peninsular Malaysia was observed to have slightly higher diversity (CDS p: 0.01307) than those from Malaysian Borneo (CDS p: 0.01212). Natural selection analysis on PkSPATR indicated significant purifying selection. Multiple amino acid sequence alignment revealed 69 polymorphic sites. The phylogenetic tree and haplotype network did not show any distinct clustering of PkSPATR. The low genetic diversity level, natural selection and absence of clustering implied functional constrains of the PkSPATR protein.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education Malaysia Long Term Research Grant Scheme [LRGS/1/2018/UM/01/1/1] |
| Uncontrolled Keywords: | Plasmodium knowlesi; SPATR; genetic diversity; haplotype analysis |
| Subjects: | R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Koh Ai Peng |
| Date Deposited: | 06 Nov 2024 09:06 |
| Last Modified: | 06 Nov 2024 09:06 |
| URI: | http://eprints.um.edu.my/id/eprint/46126 |
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