Windarsih, Anjar and Abu Bakar, Nor Kartini and Rohman, Abdul and Erwanto, Yuny (2024) Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study. Analytical Sciences, 40 (3, SI). pp. 385-397. ISSN 0910-6340, DOI https://doi.org/10.1007/s44211-023-00470-x.
Full text not available from this repository.Abstract
Due to the different price and high quality, halal meat such as beef can be adulterated with non-halal meat with low price to get an economical price. The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a non-targeted metabolomics approach employing liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics. The differentiation of authentic BM from that adulterated with DM was successfully performed using partial least square-discriminant analysis (PLS-DA) with high accuracy ((RX)-X-2 = 0.980, and (RY)-Y-2 = 0.980) and good predictivity (Q(2) = 0.517). In addition, partial least square (PLS) and orthogonal PLS (OPLS) were successfully used to predict the DM added (% w/w) in BM with high accuracy (R-2 > 0.990). A number of metabolites, potential for biomarker candidates, were identified to differentiate BM and that adulterated with DM. It showed that the combination of a non-targeted LC-HRMS Orbitrap metabolomics and chemometrics could detect up to 0.1% w/w of DM adulteration. The developed method was successfully applied for analysis of commercial meatball samples (n = 28). Moreover, pathway analysis revealed that beta-alanine, histidine, and ether lipid metabolism were significantly affected by dog meat adulteration. In summary, this developed method has great potential to be developed and used as an alternative method for analysis of non-halal meats in halal meat products.
| Item Type: | Article |
|---|---|
| Funders: | Kementerian Pendidikan, Kebudayaan, Riset, dan Teknologi, Lembaga Pengelola Dana Pendidikan (LPDP), Ministry of Finance, Republic of Indonesia |
| Uncontrolled Keywords: | Beef meatballs; Chemometrics; Dog meat; LC-HRMS; Non-targeted metabolomics |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 15 Nov 2024 01:03 |
| Last Modified: | 15 Nov 2024 01:03 |
| URI: | http://eprints.um.edu.my/id/eprint/45952 |
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