Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B)

Tee, K.K. and Kusagawa, S. and Li, X.J. and Onogi, N. and Isogai, M. and Hase, S. and Uenishi, R. and Liao, H.N. and Kamarulzaman, A. and Takebe, Y. (2009) Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B). PLoS ONE, 4 (8). e6666. ISSN 1932-6203,

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Abstract

A growing number of emerging HIV-1 recombinants classified as circulating recombinant forms (CRFs) have been identified in Southeast Asia in recent years, establishing a molecular diversity of increasing complexity in the region. Here, we constructed a replication-competent HIV-1 clone for CRF3301B (designated p05MYKL045.1), a newly identified recombinant comprised of CRF01AE and subtype B. p05MYKL045.1 was reconstituted by cloning of the near full-length HIV-1 sequence from a newly-diagnosed individual presumably infected heterosexually in Kuala Lumpur, Malaysia. The chimeric clone, which contains the 59 LTR (long terminal repeat) region of p93JP-NH1 (a previously isolated CRF01AE infectious clone), showed robust viral replication in the human peripheral blood mononuclear cells. This clone demonstrated robust viral propagation and profound syncytium formation in CD4(+), CXCR4-expressing human glioma NP-2 cells, indicating that p05MYKL045.1 is a CXCR4-using virus. Viral propagation, however, was not detected in various human T cell lines including MT-2, M8166, Sup-T1, H9, Jurkat, Molt-4 and PM1.p05MYKL045.1 appears to proliferate only in restricted host range, suggesting that unknown viral and/or cellular host factors may play a role in viral infectivity and replication in human T cell lines. Availability of a CRF3301B molecular clone will be useful in facilitating the development of vaccine candidates that match the HIV-1 strains circulating in Southeast Asia.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Tee, Kok Keng Kusagawa, Shigeru Li, Xiao-Jie Onogi, Narumi Isogai, Maya Hase, Saiki Uenishi, Rie Liao, Huanan Kamarulzaman, Adeeba Takebe, Yutaka
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 25 Jan 2013 01:47
Last Modified: 13 Nov 2017 07:33
URI: http://eprints.um.edu.my/id/eprint/4594

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