Manimaran, Malarmugila and Teo, Yin Yin and Kah, James Chen Yong and Beishenaliev, Adilet and Loke, Yean Leng and Foo, Yiing Yee and Ng, Shiow-Fern and Chee, Chin Fei and Chin, Sek Peng and Faruqu, Farid Nazer and Chang, Chia-Yu and Misran, Misni and Chung, Lip Yong and Leo, Bey Fen and Chiou, Shih-Hwa and Chang, Chia-Ching and Tay, Sun Tee and Kiew, Lik Voon (2024) PDADMAC/Alginate-Coated Gold Nanorod For Eradication of Staphylococcus Aureus Biofilms. International Journal of Nanomedicine, 19. pp. 3697-3714. ISSN 1178-2013, DOI https://doi.org/10.2147/IJN.S452085.
Full text not available from this repository.Abstract
Introduction: Over 75% of clinical microbiological infections are caused by bacterial biofilms that grow on wounds or implantable medical devices. This work describes the development of a new poly(diallyldimethylammonium chloride) (PDADMAC)/alginatecoated gold nanorod (GNR/Alg/PDADMAC) that effectively disintegrates the biofilms of Staphylococcus aureus ( S. aureus ), a prominent pathogen responsible for hospital-acquired infections. Methods: GNR was synthesised via seed-mediated growth method, and the resulting nanoparticles were coated first with Alg and then PDADMAC. FTIR, zeta potential, transmission electron microscopy, and UV-Vis spectrophotometry analysis were performed to characterise the nanoparticles. The efficacy and speed of the non-coated GNR and GNR/Alg/PDADMAC in disintegrating S. aureus - preformed biofilms, as well as their in vitro biocompatibility (L929 murine fibroblast) were then studied. Results: The synthesised GNR/Alg/PDADMAC (mean length: 55.71 +/- 1.15 nm, mean width: 23.70 +/- 1.13 nm, aspect ratio: 2.35) was biocompatible and potent in eradicating preformed biofilms of methicillin-resistant (MRSA) and methicillin-susceptible S. aureus (MSSA) when compared to triclosan, an antiseptic used for disinfecting S. aureus colonisation on abiotic surfaces in the hospital. The minimum biofilm eradication concentrations of GNR/Alg/PDADMAC (MBEC 50 for MRSA biofilm = 0.029 nM; MBEC 50 for MSSA biofilm = 0.032 nM) were significantly lower than those of triclosan (MBEC 50 for MRSA biofilm = 10,784 nM; MBEC 50 for MRSA biofilm 5967 nM). Moreover, GNR/Alg/PDADMAC was effective in eradicating 50% of MRSA and MSSA biofilms within 17 min when used at a low concentration (0.15 nM), similar to triclosan at a much higher concentration (50 mu M). Disintegration of MRSA and MSSA biofilms was confirmed by field emission scanning electron microscopy and confocal laser scanning microscopy. Conclusion: These findings support the potential application of GNR/Alg/PDADMAC as an alternative agent to conventional antiseptics and antibiotics for the eradication of medically important MRSA and MSSA biofilms.
| Item Type: | Article |
|---|---|
| Funders: | Impact-oriented Interdisciplinary Research Grant (IIRG) from the Universiti Malaya (IIRG003B-19FNW); (IIRG003C-19FNW), Ministry of Education, Malaysia, National Science and Technology Council (NSTC) , Taiwan, ROC (NSTC 112-2321-B-A49-005); (NSTC 112-2927-I-A49-001), Ministry of Education through the SPROUT Project and the Center for Intelligent Drug Systems and Smart Biodevices (IDS 2 B) of NYCU, Taiwan, ROC (NSTC 111-2112-M-A49-025); (MSTC 110-2923-M-009-005-MY3); (NSTC 111-2927-I-A49-004) |
| Uncontrolled Keywords: | biofilm; gold nanorod; S. aureus; PDADMAC; MRSA; MSSA |
| Subjects: | Q Science > QD Chemistry R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Medicine Faculty of Medicine > Department of Molecular Medicine Faculty of Medicine > Medical Microbiology Department Faculty of Science > Department of Chemistry Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre Faculty of Pharmacy > Department of Pharmaceutical Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 13 Nov 2024 09:01 |
| Last Modified: | 13 Nov 2024 09:01 |
| URI: | http://eprints.um.edu.my/id/eprint/45878 |
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