Lau, K.A. and Wang, B. and Kamarulzaman, A. and Ng, K.P. and Saksena, N.K. (2008) Continuous crossover(s) events of HIV-1CRF01AE and B subtype strains in Malaysia: evidence of rapid and extensive HIV-1 evolution in the region. Current Hiv Research, 6 (2). pp. 108-116. ISSN 1570-162X,
Full text not available from this repository.Abstract
The Asian HIV epidemic appears to be complex, characterized by the prevalence of multiple subtypes and circulating recombinant forms with gradual replacement of pure HIV-1 subtypes in several geographical regions. The main objectives of the present study are to identify and analyse the full-length viral genomes of three unique recombinant forms (URFs); the HIV-1 isolates 07MYKLD47, 07MYKLD48 and 07MYKLD49 from Malaysia. Long-range polymerase chain reaction (PCR) amplification of seven overlapping reading frames was used to derive near full-length HIV-1 genomes. Detailed phylogenetic and bootscanning analyses were performed to determine phylogenetic associations and subtypic assignments. We further confirmed the mosaic composition of these CRF01AE/B inter-subtype recombinant forms, which are composed of B-subtype fragment(s) in the backbone of CRF01AE. Both 07MYKLD47 and 07MYKLD48 have an insertion of B subtype (880 bp and 532 bp) in the gag-pol and gp41-env gene regions, respectively. Whereas the isolate 07MYKLD49 has three B-subtype fragments inserted in different gene region along the genome; one each in the gag-pol (1862 bp) and pol-vif (1935 bp) regions, and a short B-subtype insertion (541 bp) in the 5' LTR-gag region. This highlights the public health relevance of newly emerging second generation HIV-1 recombinant forms and their dispersal, along with their rapid and continuous evolution in the region.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Lau, Katherine A. Wang, Bin Kamarulzaman, Adeeba Ng, Kee-Peng Saksena, Nitin K. |
| Uncontrolled Keywords: | HIV-1; CRF01AE/B inter-subtype recombinant; CRF01AE; subtype B; unique recombinant form; Malaysia |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms azrahani halim |
| Date Deposited: | 25 Jan 2013 01:19 |
| Last Modified: | 25 Jan 2013 01:19 |
| URI: | http://eprints.um.edu.my/id/eprint/4558 |
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