Wang, B. and Lau, K.A. and Ong, L.Y. and Shah, M. and Steam, M.C. and Foley, B. and Dwyer, D.E. and Chew, C.B. and Kamarulzaman, A. and Ng, K.P. and Saksena, N.K. (2007) Complex patterns of the HIV-1 epidemic in Kuala Lumpur, Malaysia: Evidence for expansion of circulating recombinant form CRF3301B and detection of multiple other recombinants. Virology, 367 (2). pp. 288-297. ISSN 0042-6822,
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Abstract
The HIV protease-reverse transcriptase (PR-RT) (1047 bp), gp120-env (891 bp) and gp41-env (547 bp) regions from the plasma of 115 HIV-1-infected patients in Kuala Lumpur (KL), Malaysia were sequenced. Detailed phylogenetic and bootscanning analyses were performed to determine the mosaic structure of the HIV-1 strains and their recombination breakpoint(s). Among the 50 patient samples in which all three regions could be amplified, the HIV-1 CRF0 1AE subtype (46) was predominant followed by subtypes B (10) and B' (6). A total of 9/50 (18) patients were infected with a CR-F01AE/B inter-subtype recombinant, displaying a recombinant form (RF) PR-RT, CPF01AE(gp120-env) and CRF01AE(gp41-evn). This RF was derived from the Thai variants of CRF01AE and B' subtype, with two distinct B' subtype segments in the backbone of CRF01AE, similar to the newly identified CRF33-01 B. In addition, one sample demonstrated a close structural relationship with the new CRF3301B in the PR-RT region but displayed B' segment in part of the env region (RFPR-RT, CRF01AE/B'(gp120-env)) and B'(gp41-env)) indicating continuing evolution of CRF3301B. The remaining 18 of samples were identified as unique recombinant forms (URFs). (c) 2007 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Wang, Bin Lau, Katherine A. Ong, Lai-Yee Shah, Meet Steam, Megan C. Foley, Brian Dwyer, Dominic E. Chew, Choo Beng Kamarulzaman, Adeeba Ng, Kee Peng Saksena, Nitin K. |
| Uncontrolled Keywords: | Malaysia; HIV; AIDS; Recombination; Circulating recombinant forms; Epidemiology |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms azrahani halim |
| Date Deposited: | 16 Jan 2013 02:21 |
| Last Modified: | 16 Jan 2013 02:21 |
| URI: | http://eprints.um.edu.my/id/eprint/4557 |
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