Ugusman, Azizah and Ismail, Siti Marjiana and Nor Hisam, Nur Syahidah and Hui, Chua Kien and Saleh, Mohammed S. M. and Abdul Karim, Abdul Kadir and Othman, Nur Syakirah and Hamid, Adila A. and Aminuddin, Amilia (2024) Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells. International Journal of Molecular Sciences, 25 (5). p. 2806. ISSN 1661-6596, DOI https://doi.org/10.3390/ijms25052806.
Full text not available from this repository.Abstract
Angiotensin-converting enzyme (ACE) plays a crucial role in the pathogenesis of hypertension. Piper sarmentosum Roxb., an herb known for its antihypertensive effect, lacks a comprehensive understanding of the mechanism underlying its antihypertensive action. This study aimed to elucidate the antihypertensive mechanism of aqueous extract of P. sarmentosum leaves (AEPS) via its modulation of the ACE pathway in phorbol 12-myristate-13-acetate (PMA)-induced human umbilical vein endothelial cells (HUVECs). HUVECs were divided into five groups: control, treatment with 200 mu g/mL AEPS, induction 200 nM PMA, concomitant treatment with 200 nM PMA and 200 mu g/mL AEPS, and treatment with 200 nM PMA and 0.06 mu M captopril. Subsequently, ACE mRNA expression, protein level and activity, angiotensin II (Ang II) levels, and angiotensin II type 1 receptor (AT1R) and angiotensin II type 2 receptor (AT2R) mRNA expression in HUVECs were determined. AEPS successfully inhibited ACE mRNA expression, protein and activity, and angiotensin II levels in PMA-induced HUVECs. Additionally, AT1R expression was downregulated, whereas AT2R expression was upregulated. In conclusion, AEPS reduces the levels of ACE mRNA, protein and activity, Ang II, and AT1R expression in PMA-induced HUVECs. Thus, AEPS has the potential to be developed as an ACE inhibitor in the future.
| Item Type: | Article |
|---|---|
| Funders: | Faculty of Medicine, Universiti Kebangsaan Malaysia |
| Uncontrolled Keywords: | angiotensin converting enzyme; hypertension; human umbilical vein endothelial cells; phorbol 12-myristate-13-acetate; Piper sarmentosum |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Oct 2024 02:45 |
| Last Modified: | 28 Oct 2024 02:45 |
| URI: | http://eprints.um.edu.my/id/eprint/45535 |
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