Wang, Hui and Liu, Kuiyan and Chen, Ziyan and Chen, Yang and Hu, Zhangxi and Chen, Weizhou and Leaw, Chui Pin and Chen, Nansheng (2024) Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing. Marine Pollution Bulletin, 201. p. 116198. ISSN 0025-326X, DOI https://doi.org/10.1016/j.marpolbul.2024.116198.
Full text not available from this repository.Abstract
Metabarcoding analysis is an effective technique for monitoring the domoic acid-producing Pseudo-nitzschia species in marine environments, uncovering high-levels of molecular diversity. However, such efforts may result in the overinterpretation of Pseudo-nitzschia species diversity, as molecular diversity not only encompasses interspecies and intraspecies diversities but also exhibits extensive intragenomic variations (IGVs). In this study, we analyzed the V4 region of the 18S rDNA of 30 strains of Pseudo-nitzschia multistriata collected from the coasts of China. The results showed that each P. multistriata strain harbored about a hundred of unique 18S rDNA V4 sequence varieties, of which each represented by a unique amplicon sequence variant (ASV). This study demonstrated the extensive degree of IGVs in P. multistriata strains, suggesting that IGVs may also present in other Pseudo-nitzschia species and other phytoplankton species. Understanding the scope and levels of IGVs is crucial for accurately interpreting the results of metabarcoding analysis.
| Item Type: | Article |
|---|---|
| Funders: | National Key Research and Development (R&D) Program of China (2022YFC3105200), Taishan Scholar Project Special Fund, National Natural Science Foundation of China (NSFC) (42176162), Chinese Academy of Sciences (XDB42000000), Chinese Academy of Sciences Pioneer Hundred Talents Program |
| Uncontrolled Keywords: | Metabarcoding analysis; Pseudo-nitzschia multistriata; Molecular markers polymorphism; SSU rDNA; Intragenomic variations (IGVs) |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 16 Oct 2024 07:17 |
| Last Modified: | 16 Oct 2024 07:17 |
| URI: | http://eprints.um.edu.my/id/eprint/45388 |
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