Yap, Phui-Chyng and Chai, Lay Ching and Hassandarvish, Pouya and Lee, Hai Yen (2024) Phenotypic microarray characterization of hemolytic and non-hemolytic strains of Listeria monocytogenes. LWT-Food Science and Technology, 199. p. 116154. ISSN 0023-6438, DOI https://doi.org/10.1016/j.lwt.2024.116154.
Full text not available from this repository.Abstract
Limited information is available on the phenotypic characteristics of Listeria monocytogenes that may affect its virulence of L. monocytogenes. The study focused on comparing the differences between two strains of L. monocytogenes, one hemolytic (LLO+) and one non-hemolytic (tLLO). Using Phenotype Microarray (PM) technology, carbon, nitrogen, and phosphorus-sulfur source utilization by the strains was performed and the KEGG pathway involvement in substrate utilization was analyzed. Of the 285 substrates screened, the results suggested that D-gluconic acid and L-serine may play a role in L. monocytogenes hemolytic activity towards LLO production by generating pyruvate as an intermediate and amino acid biosynthesis, which may play an important role in virulence protein translation. D-gluconic acid and L-serine showed no effect on the tLLO strain in PM analysis and validation tests. Notably, the scope of this study was limited to these two strains of L. monocytogenes. While these findings provide valuable insights into the metabolic differences between hemolytic and nonhemolytic strains, further research is necessary to expand this understanding to a broader range of strains and explore additional factors that may influence LLO functionality.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia (FRGS/1/2018/STG03/UM/02/12) ; (FP049-2018A), Bantuan Kecil Pendidikan (BKP) (BK056-2016), Higher Institution Center for Excellence (HICoE) program (MO002-2019) |
| Uncontrolled Keywords: | Listeria monocytogenes; Food safety; Listeriolysin O; Pathogenicity; Biosafety; Phenotype microarray |
| Subjects: | Q Science > QR Microbiology R Medicine |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 07 Oct 2024 04:41 |
| Last Modified: | 07 Oct 2024 04:41 |
| URI: | http://eprints.um.edu.my/id/eprint/45283 |
Actions (login required)
 |
View Item |
