Lai, Meng Yee and Abdullah, Mohd Lutfi and Lau, Yee Ling (2024) Real-time fluorescence loop-mediated isothermal amplification assays for detection of zoonotic malaria Plasmodium parasites. Acta Tropica, 255. p. 107249. ISSN 0001-706X, DOI https://doi.org/10.1016/j.actatropica.2024.107249.
Full text not available from this repository.Abstract
Background: Natural human infections by Plasmodium cynomolgi and P. inui have been reported recently and gain the substantial attention from Southeast Asian countries. Zoonotic transmission of non -human malaria parasites to humans from macaque monkeys occurred through the bites of the infected mosquitoes. The objective of this study is to establish real-time fluorescence loop -mediated isothermal amplification (LAMP) assays for the detection of zoonotic malaria parasites by combining real-time fluorescent technology with the isothermal amplification technique. Methods: By using 18S rRNA as the target gene, the primers for P. cynomolgi, P. coatneyi and P. inui were newly designed in the present study. Four novel real-time fluorescence LAMP assays were developed for the detection of P. cynomolgi, P. coatneyi, P. inui and P. knowlesi . The entire amplification process was completed in 60 min, with the assays performed at 65 degrees C. By using SYTO-9 as the nucleic acid intercalating dye, the reaction was monitored via real-time fluorescence signal. Results: There was no observed cross -reactivity among the primers from different species. All 70 field -collected monkey samples were successfully amplified by real-time fluorescence LAMP assays. The detection limit for P. cynomolgi, P. coatneyi and P. knowlesi was 5 x 10 9 copies/ mu L. Meanwhile, the detection limit of P. inui was 5 x 10 10 copies/ mu L. Conclusion: This is the first report of the detection of four zoonotic malaria parasites by real-time fluorescence LAMP approaches. It is an effective, rapid and simple -to -use technique. This presented platform exhibits considerable potential as an alternative detection for zoonotic malaria parasites.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education Malaysia Long Term Research Grant Scheme (LRGS/1/2018/UM/01/1/4) |
| Uncontrolled Keywords: | Primates; Zoonotic malaria; P. cynomolgi; P. coatneyi; P. inui; P. knowlesi LAMP |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Sep 2024 08:19 |
| Last Modified: | 18 Sep 2024 08:19 |
| URI: | http://eprints.um.edu.my/id/eprint/45133 |
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