Lee, Siew Wei and Logis, Joko and Fatima, Ayesha and Lim, Renee Lay Hong and Ng, Szu Ting and Tan, Chon Seng and Fung, Shin Yee and Tan, Nget Hong and Goh, Amos Eng Liang and Tan, Yong Hui and Lim, Crystale Siew Ying (2022) Dye decolorisation capacity of lignosus rhinocerotis (cooke) ryvarden dialysed fraction and its expression of novel recombinant laccase in pichia pastoris. Malaysian Journal of Biochemistry and Molecular Biology, 25 (1). 104 -113. ISSN 1511-2616,
Full text not available from this repository.Abstract
Azo dyes are widely used in the textile industry due to their bold colors and resistance toward degradation. This has made azo dyes the global burden in wastewater treatment as conventional dye removal methods have their limitations. Laccase has emerged as an alternative due to its ability to decolorise a wide range of dyes, producing water as by-product. In this study, we aim to assess the dye decolorisation capacity of the Basidiomycota, L. rhinocerotis dialysed fraction and produce recombinant laccase expressed in Pichia pastoris. Out of the 3 dyes tested, the dialysed fraction of L. rhinocerotis sclerotia was capable of decolorising around 90 of the Congo Red and Coomassie Brilliant Blue dye at the enzyme unit of 0.018 U/mL. The gDNA of Lac1 MG210944 was successfully updated in GenBank and the sequence was used to design the pPICZαA/Lac vector, which was successfully cloned and the recombinant Lac1 was successfully expressed using the Pichia pastoris system. The recombinant Lac1 expressed contain the 6x His-tag for purification but the binding towards the Ni-NTA resin was weak, therefore the purification can be optimised through reducing Imidazole concentration to compensate for the weak binding. In conclusion, L. rhinocerotis extract showed promising results in decolorising CR and CBB, while recombinant Lac1 laccase successfully demonstrated its activity and can be further used for dye decolorisation in future studies. © 2022 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | Ligno Biotech Sdn, UCSI [Proj-In-FAS-021], Malaysia Toray Science Foundation [TML-CA/15.205] |
| Uncontrolled Keywords: | Azo dye; Dye decolorisation; Expression; Laccase; Lignosus rhinocerotis |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Medicine > Department of Molecular Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 09 Jul 2024 06:41 |
| Last Modified: | 09 Jul 2024 06:41 |
| URI: | http://eprints.um.edu.my/id/eprint/43889 |
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