Early activation of the interleukin-23-17 axis in a murine model of oropharyngeal candidiasis

Saunus, J.M. and Wagner, S.A. and Matias, M.A. and Hu, Y. and Zaini, Zuraiza Mohamad and Farah, C.S. (2010) Early activation of the interleukin-23-17 axis in a murine model of oropharyngeal candidiasis. Molecular Oral Microbiology, 25 (5). pp. 343-356. ISSN 2041-1006, DOI https://doi.org/10.1111/j.2041-1014.2010.00570.x.

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Official URL: https://doi.org/10.1111/j.2041-1014.2010.00570.x

Abstract

P>Candida albicans is an oral commensal yeast that causes oropharyngeal candidiasis (OPC) in immunocompromised individuals. The immunological pathways involved in OPC have been revisited after the interleukin-17 (IL-17) pathway was implicated in fungal immunity. We studied immediate (< 24 h) and adaptive (3-6 day) IL-12 and IL-23-17 pathway activation in naive p40-/- mice, which lack IL-12 and IL-23 and develop severe, chronic OPC upon oral inoculation with C. albicans. Macrophages from p40-/- mice were less efficient than C57BL/6J controls at killing C. albicans in vitro but very low numbers in the oral mucosae of infected C57BL/6J mice suggest that they are not critical in vivo, at least in this strain. Migration of macrophages to regional lymph nodes of infected p40-/- mice was impaired; however, dendritic cell migration was not affected. Recombinant IL-12 therapy provided only temporary relief from OPC, suggesting that IL-23 is required for full protection. In C57BL/6J mice, but not p40-/- mice, messenger RNAs encoding IL-23p19 and IL-17 were induced in the oral mucosa within 24 h of infection (6 +/- 0.6 and 12 +/- 2.7-fold). By day 6 of infection in C57BL/6J mice, IL-17A messenger RNA level had increased 5.1 +/- 1.8 and 83 +/- 21-fold in regional lymph nodes and oral tissues respectively. Ablation of p40 was associated with delayed or abrogated induction of IL-17A pathway targets (monocyte chemoattractant protein-1, IL-6 and macrophage inflammatory protein-2), and a lack of organized recruitment of neutrophils to the infected oral mucosa. Overall our data show that the IL-23-17A axis is activated early in the oral mucosae of immunologically naive mice with OPC.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: ISI Document Delivery No.: 646OK Times Cited: 4 Cited Reference Count: 55 Cited References: Acosta-Rodriguez EV, 2007, NAT IMMUNOL, V8, P639, DOI 10.1038/ni1467 Ashman RB, 2004, IMMUNOL CELL BIOL, V82, P196, DOI 10.1046/j.0818-9641.2004.01217.x Bastos KRB, 2004, MICROBES INFECT, V6, P630, DOI 10.1016/j.micinf.2004.02.012 Bastos KRB, 2002, J LEUKOCYTE BIOL, V71, P271 Beklen A, 2009, ORAL MICROBIOL IMMUN, V24, P38, DOI 10.1111/j.1399-302X.2008.00473.x Conti HR, 2009, J EXP MED, V206, P299, DOI 10.1084/jem.20081463 Decken K, 1998, INFECT IMMUN, V66, P4994 Dongari-Bagtzoglou A, 2005, MED MYCOL, V43, P545, DOI 10.1080/13693780500064557 d'Ostiani CF, 2000, J EXP MED, V191, P1661, DOI 10.1084/jem.191.10.1661 Eyerich K, 2008, J INVEST DERMATOL, V128, P2640, DOI 10.1038/jid.2008.139 Farah CS, 2006, ORAL MICROBIOL IMMUN, V21, P252, DOI 10.1111/j.1399-302X.2006.00288.x Farah CS, 2002, INFECT IMMUN, V70, P724, DOI 10.1128/IAI.70.2.724-731.2002 Farah CS, 2001, INFECT IMMUN, V69, P6110, DOI 10.1128/IAI.69.10.6110-6118.2001 Farah CS, 2000, CLIN DERMATOL, V18, P553, DOI 10.1016/S0738-081X(00)00145-0 Farah CS, 2009, ORAL MICROBIOL IMMUN, V24, P83, DOI 10.1111/j.1399-302X.2008.00462.x Fidel PL, 2002, ORAL DIS, V8, P69, DOI 10.1034/j.1601-0825.2002.00015.x Filler SG, 2006, CURR OPIN MICROBIOL, V9, P333, DOI 10.1016/j.mib.2006.06.005 Frucht DM, 2001, TRENDS IMMUNOL, V22, P556, DOI 10.1016/S1471-4906(01)02005-1 Gerosa F, 2008, J EXP MED, V205, P1447, DOI 10.1084/jem.20071450 Harrington LE, 2005, NAT IMMUNOL, V6, P1123, DOI 10.1038/ni1254 Hu Y, 2006, IMMUNOL CELL BIOL, V84, P455, DOI 10.1111/j.1440-1711.2006.01457.x Huang WT, 2004, J INFECT DIS, V190, P624, DOI 10.1086/422329 Kleinschek MA, 2006, J IMMUNOL, V176, P1098 LeibundGut-Landmann S, 2007, NAT IMMUNOL, V8, P630, DOI 10.1038/ni1460 Ley K, 2006, IMMUNOL RES, V34, P229, DOI 10.1385/IR:34:3:229 Liang SC, 2006, J EXP MED, V203, P2271, DOI 10.1084/jem.20061308 Lockhart E, 2006, J IMMUNOL, V177, P4662 Ma CS, 2008, J EXP MED, V205, P1551, DOI 10.1084/jem.20080218 Magram J, 1996, IMMUNITY, V4, P471, DOI 10.1016/S1074-7613(00)80413-6 MARTINO P, 1994, EUR J CLIN MICROBIOL, V13, P797, DOI 10.1007/BF02111339 Matsuzaki G, 2007, MICROBIOL IMMUNOL, V51, P1139 Milner JD, 2008, NATURE, V452, P773, DOI 10.1038/nature06764 O'Brien RL, 2009, EUR J IMMUNOL, V39, P662, DOI 10.1002/eji.200839120 Pirofski LA, 2009, J EXP MED, V206, P269, DOI 10.1084/jem.20090093 Romagnoli G, 2004, J LEUKOCYTE BIOL, V75, P117, DOI 10.1189/jlb.0503226 Romani Luigina, 2004, Nat Rev Immunol, V4, P1 Rudner XL, 2007, INFECT IMMUN, V75, P3055, DOI 10.1128/IAI.01329-06 Saunus JM, 2008, FRONT BIOSCI, V13, P5345, DOI 10.2741/3085 Schaller M, 2004, MICROBIOL-SGM, V150, P2807, DOI 10.1099/mic.0.27169-0 Peltroche-Llacsahuanga H, 2000, FEMS MICROBIOL LETT, V191, P151, DOI 10.1016/S0378-1097(00)00384-0 Shen F, 2008, CYTOKINE, V41, P92, DOI 10.1016/j.cyto.2007.11.013 Shibata K, 2007, J IMMUNOL, V178, P4466 Sims CR, 2005, ARCH MED RES, V36, P660, DOI 10.1016/j.arcmed.2005.05.015 Trinchieri G, 2003, NAT REV IMMUNOL, V3, P133, DOI 10.1038/nri1001 Uhlig HH, 2006, IMMUNITY, V25, P309, DOI 10.1016/j.immuni.2006.05.017 van der Graaf CAA, 2005, INFECT IMMUN, V73, P7458, DOI 10.1128/IAI.73.11.7458-7464.2005 Vonk AG, 2002, J MICROBIOL METH, V49, P55, DOI 10.1016/S0167-7012(01)00348-7 Weaver CT, 2007, ANNU REV IMMUNOL, V25, P821, DOI 10.1146/annurev.immunol.25.022106.141557 Weindl G, 2007, J CLIN INVEST, V117, P3664, DOI 10.1172/JCI28115 Ye P, 2001, J EXP MED, V194, P519, DOI 10.1084/jem.194.4.519 Yu JJ, 2008, FRONT BIOSCI-LANDMRK, V13, P170, DOI 10.2741/2667 Zelante T, 2007, EUR J IMMUNOL, V37, P2695, DOI 10.1002/eji.200737409 Zhang XY, 2009, J IMMUNOL, V183, P1291, DOI 10.4049/jimmunol.0803075 Zhou MH, 2008, IMMUNOL LETT, V118, P72, DOI 10.1016/j.imlet.2008.03.004 Zhou P, 1997, INFECT IMMUN, V65, P936 Saunus, J. M. Wagner, S. A. Matias, M. A. Hu, Y. Zaini, Z. M. Farah, C. S. Australian Dental Research Foundation This work was funded by the Australian Dental Research Foundation. The authors acknowledge the technical assistance of and valuable discussions with Geoff Osborne and Virginia Nink at the UQ Qld Brain Institute Flow Cytometry Facility, and are grateful to Anthony Chan for assistance with immunohistochemical staining. Wiley-blackwell Malden
Uncontrolled Keywords: Candida albicans; Interleukin 23; Interleukin 17A; Oropharyngeal candidiasis
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 10 Jan 2013 00:40
Last Modified: 25 Oct 2018 07:41
URI: http://eprints.um.edu.my/id/eprint/4366

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