Siar, C.H. and Kawakami, T. and Buery, R.R. and Nakano, K. and Tomida, M. and Tsujigiwa, H. and Han, P.P. and Nagatsuka, H. and Ng, K.H. (2011) Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor. European Journal of Medical Research, 16 (11). pp. 501-506. ISSN 0949-2321,
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Abstract
Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs) are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites). Although GCs are present in a variety of oclontogenic lesions notably the calcifying cystic odontogenic tumor (CCOT), their nature and process of formation remains elusive. The aim of this study was to investigate the role of Notch signaling in the cell fate specification of GCs in CCOT. Immunohistochemical staining for four Notch receptors (Notchl, Notch2, Notch3 and Notch4) and three ligands (jagged1, jagged2 and Delta)) was performed on archival tissues of five CCOT cases. Level of positivity was quantified as negative (0), mild (+), moderate (2+) and strong (3+). Results revealed that GCs demonstrated overexpression for Notchl and jaggedl suggesting that Notchljaggedl signaling might serve as the main transduction mechanism in cell fate decision for GCs in CCOT. Protein localizations were largely membranous and/or cytoplasmic. Mineralized GCs also stained positive implicating that the calcification process might be associated with upregulation of these molecules. The other Notch receptors and lig-ands were weak to absent in GCs and tumoral epithelium. Stromal endothelium and fibroblasts were stained variably positive.
| Item Type: | Article |
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| Funders: | UNSPECIFIED |
| Additional Information: | ISI Document Delivery No.: 861ZH Times Cited: 0 Cited Reference Count: 27 Cited References: Artavanis-Tsakonas S, 1999, SCIENCE, V284, P770, DOI 10.1126/science.284.5415.770 BARNES L, 2005, WHO CLASSIFICATION T, P302 Bray S, 1998, SEMIN CELL DEV BIOL, V9, P591, DOI 10.1006/scdb.1998.0262 CHITNIS AB, 1995, MOL CELL NEUROSCI, V6, P311, DOI 10.1006/mcne.1995.1024 Chuah KS, 2009, J HARD TISSUE BIOL, V18, P63 Fernandez-Rodriguez J, 2011, HUM MUTAT, V32, P705, DOI 10.1002/humu.21500, 10.1002/hed.21771 GORLIN R J, 1962, Oral Surg Oral Med Oral Pathol, V15, P1235, DOI 10.1016/0030-4220(62)90159-7 KEREBEL B, 1985, ORAL SURG ORAL MED O, V59, P371, DOI 10.1016/0030-4220(85)90062-3 KIM J, 2000, ORAL SURG ORAL MED O, V90, pE630 Leong KG, 2006, BLOOD, V107, P2223, DOI 10.1182/blood-2005-08-3329 Lucchese A, 2007, ORAL SURG ORAL MED O, V104, P391, DOI 10.1016/j.tripleo.2006.09.005 Matsuzaka K, 2001, Bull Tokyo Dent Coll, V42, P51, DOI 10.2209/tdcpublication.42.51 MITSIADIS TA, 1995, J CELL BIOL, V130, P407, DOI 10.1083/jcb.130.2.407 Nagatsuka H, 2002, VIRCHOWS ARCH, V441, P392, DOI 10.1007/s00428-002-0658-1 Nakano K, 2010, J HARD TISSUE BIOL, V19, P147, DOI 10.2485/jhtb.19.147 Nakano K, 2002, J ORAL PATHOL MED, V31, P494, DOI 10.1034/j.1600-0714.2002.00162.x NG KH, 1985, ORAL SURG ORAL MED O, V60, P417, DOI 10.1016/0030-4220(85)90265-8 Philipsen HP, 2007, J ORAL PATHOL MED, V36, P383, DOI 10.1111/j.1600-0714.2007.00536.x Ross DA, 2004, EXP CELL RES, V296, P173, DOI 10.1016/j.yexer.2004.02.003 SIAR CH, 1988, BRIT J ORAL MAX SURG, V26, P215, DOI 10.1016/0266-4356(88)90165-9 Siar CH, 2010, J HARD TISSUE BIOL, V19, P167 SIAR CH, 1993, BRIT J ORAL MAX SURG, V31, P183, DOI 10.1016/0266-4356(93)90122-D SIAR CH, 1987, INT J ORAL MAX SURG, V16, P95, DOI 10.1016/S0901-5027(87)80036-X Siar CH, 2010, ORAL SURG ORAL MED O, V110, P224, DOI 10.1016/j.tripleo.2010.03.009 Siar CH, 2010, J ORAL PATHOL MED, V39, P552, DOI 10.1111/j.1600-0714.2009.00871.x Siar CH, 2010, EUR J MED RES, V15, P180 SONONE A, 2011, ORAL SURG ORAL MED O, V4, P77 Siar, C. H. Kawakami, T. Buery, R. R. Nakano, K. Tomida, M. Tsujigiwa, H. Han, P. P. Nagatsuka, H. Ng, K. H. ERGSFP028/2010A; Ministry of Higher Education, Malaysia; Japan Society for the Promotion of Science20592349, 23592951 This study is supported by ERGS FP028/2010A, Ministry of Higher Education, Malaysia and Grant-in-aid for Scientific Research (C) (20592349 and 23592951) from the Japan Society for the Promotion of Science. I holzapfel verlag gmbh Munich |
| Uncontrolled Keywords: | Notch Jagged Delta Calcifying cystic odontogenic tumor (CCOT) Ghost cells differential expression ameloblastoma keratocyst jagged1 chains |
| Subjects: | R Medicine > RK Dentistry |
| Divisions: | Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology |
| Depositing User: | Ms Nursyafiqah Abd Malek |
| Date Deposited: | 10 Jan 2013 00:02 |
| Last Modified: | 10 Jan 2013 00:02 |
| URI: | http://eprints.um.edu.my/id/eprint/4323 |
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